We narrowed to 30,760 results for: PLE

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TB:2xP53_RE:MiniP

Plasmid

#133881

Purpose

2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

2 copies of the p53 DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_h7SK

Plasmid

#102846

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert SAM_h7SK

Use

Pcr template for stagr inserts

Promoter

H7SK

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pG418R-GMRWhite

Plasmid

#165883

Purpose

G418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b+R3(R112C/T180C)

Plasmid

#108195

Purpose

Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA3(R112C/T180C) fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup88-mut

Plasmid

#87337

Purpose

To express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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SLC9A3R2_PDZ_2

Plasmid

#103926

Purpose

Protein expression and purification of NHERF2 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SLC9A3R2 (NHERF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159851

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMDC84-SEIPIN3-Ndelta

Plasmid

#96981

Purpose

Express N-terminal truncated Arabidopsis SEIPIN3 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN3 (AT2G34380 Mustard Weed)

Tags

6xHis and GFP

Expression

Plant

Mutation

A segment of 507 nucleotides are deleted from the…

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-GW1D1a(aa254-503)

Plasmid

#21520

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa254-503

Available Since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmCaf40

Plasmid

#79251

Purpose

Expresses EGFP-tagged DmCaf40 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCaf40 (Rcd-1 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCRII-Sema4d-ISH-2kb

Plasmid

#68032

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4D (Sema4d Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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YFP(159-238) in pcDNAI/Amp

Plasmid

#60889

Purpose

This vector attaches YFP(159-238) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

Insert

YFP(159-238)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b-GB

Plasmid

#129689

Purpose

express E. coli GreB

Depositor

Jeff Gelles

Article

Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

his6/thrombin/GreB (greB E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE673

Plasmid

#153239

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-GW1D5(aa1670-1962)

Plasmid

#21519

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa1670-1962

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pscALPS-SIV-COL-BWC01 vpr

Plasmid

#115840

Purpose

Encodes codon optimized 3xFLAG-SIVCOLVpr (KF214240) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCOLVpr (KF214240)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-RCM-vpr

Plasmid

#115838

Purpose

Encodes codon optimized 3xFLAG-SIVRCM Vpr (HM803689) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVRCM Vpr (HM803689)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-SUN-K08 vpr

Plasmid

#115832

Purpose

Encodes codon optimized 3xFLAG-SIVSUN Vpr (FR751162) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVSUN Vpr (FR751162)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-GSN-CN166 vpr

Plasmid

#115831

Purpose

Encodes codon optimized 3xFLAG SIVGSN-CN166 Vpr (AF468659) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGSN-CN166 Vpr (AF468659)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-SYK vpr

Plasmid

#115821

Purpose

Encodes codon optimized 3xFLAG-SIVSYK Vpr (L06042) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVSYK Vpr (L06042)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-TAL vpr

Plasmid

#115820

Purpose

Encodes codon optimized 3xFLAG-SIVTAL Vpr (AM182197) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVTAL Vpr (AM182197)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

Plasmid

#159800

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJOE6089.4

Plasmid

#135081

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-eGFP-Strep-tag II

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159843

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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RFN_EGFP_47

Plasmid

#60071

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only
p2Flag CMV2-ZO-2-1st-PDZ-mut

Plasmid

#27416

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

K38E, F44H, I46E and V48E

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsXRN1_1174-1649_AH

Plasmid

#148903

Purpose

Mammalian Expression of HsXRN1_1174-1649

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

Insert

HsXRN1_1174-1649 (XRN1 Human)

Expression

Mammalian

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER-ARF3b

Plasmid

#67496

Purpose

Expression of shRNA targeting human ARF3b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF3 shRNA (ARF3 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-ARF4b

Plasmid

#67498

Purpose

Expression of shRNA targeting human ARF4b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF4 shRNA (ARF4 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
GFP-GW1D10(aa566-1343)

Plasmid

#21517

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa566-1343

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only