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pDEST-FRT/T0-GFP-BRCA1

Plasmid

#71116

Purpose

mammalian expression of GFP-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMXs-DsRed Express

Plasmid

#22724

Depositor

Shinya Yamanaka

Article

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

a variant of Discosoma sp. red fluorescent protein

Use

Retroviral

Expression

Mammalian

Mutation

N/A

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro GFP shRNA

Plasmid

#10675

Purpose

Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

Depositor

William Hahn

Article

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

GFP shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2-YAP2

Plasmid

#19045

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1-HA

Plasmid

#24399

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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tetO-GATA4

Plasmid

#46030

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GATA4 (GATA4 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

R43Q, G50S and N197S

Promoter

tetO

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pShuttle-Cre-HA

Plasmid

#16583

Depositor

Bert Vogelstein

Article

Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Insert

cre recombinase (cre P1 bacteriophage)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-zif268

Plasmid

#18045

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

zif268

Expression

Bacterial

Mutation

Zif268 is fused to the omega subunit of RNA polym…

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Rac1-Q61L

Plasmid

#12983

Depositor

Gary Bokoch

Insert

Rac1 constitutively active (RAC1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Q61L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pdCas9-DNMT3A-PuroR_v2

Plasmid

#74407

Purpose

Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A S. pyogenes, Human, Synthetic)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

CBh

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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