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pDEST_FLAG-HUWE1

Plasmid

#187120

Purpose

mammalian cell expression of FLAG-HUWE1

Depositor

Eric Fischer

Publication

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE5

Plasmid

#90339

Purpose

STAT3 - Stat3 homodimer gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

STAT3

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMRX-IB-HaloTag7-mGFP-KDEL

Plasmid

#184904

Purpose

Stable expression of HaloTag7-mEGFP-KDEL in mammalian cells to measure ER-phagy activity

Depositor

Noboru Mizushima

Publication

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KDEL

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMCP-RecT

Plasmid

#183827

Purpose

pLenti-EF1A-MCP-EXTEN-RecT-NLS

Depositor

Le Cong

Publication

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

MCP-RecT_NLS

Expression

Mammalian

Promoter

EF1A

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(WT)-FLAG

Plasmid

#164972

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Publication

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CoV2-N-R203M

Plasmid

#177952

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (R203M) (N )

Expression

Mammalian

Mutation

R203M

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-GCaMP6s

Plasmid

#171156

Purpose

Encodes GCaMP6s

Depositor

Hillel Adesnik

Publication

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GCaMP6s

Expression

Mammalian

Available since

Aug. 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-Sfp

Plasmid

#159617

Purpose

Expression plasmid for Sfp phosphopantetheinyl transferase

Depositor

Christopher T Walsh, Jun Yin

Publication

Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

Insert

Sfp

Tags

6HIS

Expression

Bacterial

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pC1-HyPerRed-mito

Plasmid

#60247

Purpose

Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Publication

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HyPerRed-mito

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-PE2-SpRY

Plasmid

#159979

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Publication

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PE2-SpRY

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSFDuet-sumo-NSP12 (SARS-CoV-2)

Plasmid

#159107

Purpose

The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

Depositor

Thomas Tuschl

Publication

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

NSP12

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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C1-F-tractin-mCherry

Plasmid

#155218

Purpose

Monitor global F-actin distribution

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)

Tags

mCherry

Expression

Mammalian

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pPP085

Plasmid

#158795

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Publication

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

6xHis

Expression

Bacterial

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV FLEX Synaptophysin GFP

Plasmid

#137188

Purpose

Cre-dependent expression of synaptophysin-GFP

Depositor

Matthew Nolan

Publication

Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

Insert

Synaptophysin GFP (Syp Mouse)

Use

AAV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLJM1-FLAG-GFP-Tmem192

Plasmid

#134630

Purpose

lysosomal marker

Depositor

Roberto Zoncu

Publication

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 TFAM-mClover

Plasmid

#129574

Purpose

Expression of mClover fused TFAM

Depositor

Stephen Tait

Publication

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-2E2-HA-mEGFP

Plasmid

#129595

Purpose

Encodes anti-HA frankenbody variant fused with mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Publication

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pQE30-His-PheRS

Plasmid

#124116

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-SerRS-His

Plasmid

#124118

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Seryl-tRNA ligase (serS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-LysRS-His

Plasmid

#124114

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Lysine-tRNA ligase (lysU Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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