We narrowed to 8,419 results for: BLI

1. pHAGE2-TetO-ePOUSKM

   Plasmid

   #206398

   Purpose

   Expresses four genes (human ePOU, SOX2, KLF4, c-MYC) in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Insert

   ePOU, SOX2, KLF4, c-MYC (KLF4, MYC, SOX2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-HA-FbxO11-Jeff-pcDNA3.1-

   Plasmid

   #52503

   Purpose

   expresses human FbxO11-Jeff mutant in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   FbxO11 (FBXO11 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Q491L (Jeff)

   Promoter

   CMV

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Flag-NSF/S647A

   Plasmid

   #74938

   Purpose

   Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

   Depositor

   Elisa Greggio

   Article

   Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

   Insert

   NSF (NSF Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S647A (mutation in the D2 domain of the protein)

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

4. AAV 9P31-hSyn-Venus-P2A-Tau-RING I18R/M72E

   Plasmid

   #233694

   Purpose

   AAV expression of Venus-P2A-Tau-RING-I18R/M72E from hSyn promoter

   Depositor

   Leo James

   Article

   Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

   Insert

   Venus-P2A-Tau-RING I18R-M72E
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald

   Plasmid

   #225949

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Evan Reid, Richard Eva, Bart Nieuwenhuis

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C100A

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-SFFV-CKAP4 CC-WPRE-UbC-Emerald

   Plasmid

   #225950

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutation double cysteine (CC) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Evan Reid, Richard Eva, Bart Nieuwenhuis

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Additional cysteine inserted after Cys-100

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-SFFV-CKAP4(1-131)-WPRE-UbC-Emerald

   Plasmid

   #225955

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutant lacking the luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Evan Reid, Richard Eva, Bart Nieuwenhuis

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Truncated CKAP4 (1-131) lacking the luminal domain

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-SFFV-RTN4-WPRE-UbC-Emerald

   Plasmid

   #225938

   Purpose

   Lentiviral vector plasmid expressing human reticulon 4 (RTN4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Evan Reid, Richard Eva, Bart Nieuwenhuis

   Inserts

   RTN4 (RTN4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-SFFV-myc-STIM2-WPRE-UbC-Emerald

   Plasmid

   #225940

   Purpose

   Lentiviral vector plasmid expressing human stromal interaction molecule 2 (STIM2) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Evan Reid, Richard Eva, Bart Nieuwenhuis

   Inserts

   STIM2 (STIM2 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCBE3-HF1

    Plasmid

    #218156

    Purpose

    This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-HF1
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Tags

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N497A…

    Promoter

    rpsL promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCBE3-NG

    Plasmid

    #218159

    Purpose

    This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Tags

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / L1111…

    Promoter

    rpsL promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCBE3-HF1-Hypa

    Plasmid

    #218158

    Purpose

    This plasmid harbors the base editor SCBE3-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-HF1-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Tags

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N497A…

    Promoter

    rpsL promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCBE3-Hypa

    Plasmid

    #218157

    Purpose

    This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Tags

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N692A…

    Promoter

    rpsL promoter

    Available since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Creb5 shRNA

    Plasmid

    #195020

    Purpose

    Bovine Creb5 shRNA targeting the Creb5 3′UTR

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Creb5 shRNA (CREB5 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. GAL4-Creb5 (1- 128) T59/T61 A

    Plasmid

    #195032

    Purpose

    GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with T59/T61 A mutation)

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with with T59/T61 A mutation) (CREB5 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T59/T61 A

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. GAL4-Creb5 (1- 128) C18/C23 S

    Plasmid

    #195033

    Purpose

    GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation)

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation) (CREB5 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C18/C23 S

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bn R52A, R54A pMT2

    Plasmid

    #206087

    Purpose

    Cav2.2 N-terminus (amino acids 1-95) with R52A and R54A mutations that prevent dominant-negative suppression of Cav2.2 currents by truncated Cav2.2

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R52A, R54A

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. B52_puro_gRNA_3418711_KCNB2

    Plasmid

    #197558

    Purpose

    gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)

    Depositor

    Darius Bägli

    Insert

    upstream KCNB2 promoter gRNA (Kcnb2 Rat)
    

    Use

    Grna with puromycin selection

    Tags

    puromycin resistance cassette under CMV promotion

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-pSMANTIS_SMAD-FOS_Mutant

    Plasmid

    #194188

    Purpose

    Includes the promoter (1kb) of SMTS with mutated SMAD/FOS binding site

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutated SMAD/FOS Site, Region 3-15nt of 1000nt

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-pSMANTIS_STAT1-2_Mutant

    Plasmid

    #194189

    Purpose

    Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutated STAT1/2 site, Region 653-673nt of 1000nt

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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