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pCMV-Tag/M87 S245X

Plasmid

#92368

Purpose

expression of M87 S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, S245X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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ninaE-dsocs

Plasmid

#26857

Depositor

Craig Montell

Article

Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

Insert

Slow Termination of Phototransduction (stops Fly)

Tags

myc

Expression

Insect

Mutation

This STOPS construct contains a mutated/truncated…

Available Since

April 5, 2011

Availability

Academic Institutions and Nonprofits only
pJCC_058 FnCas12a D917A

Plasmid

#171667

Purpose

For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a D917A

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Mutation

changed aspartate 917 to alanine

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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px330-UFSP2 sgRNA2

Plasmid

#134637

Purpose

contains sgRNA targeting human UFSP2 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFSP2 sgRNA2 (UFSP2 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pX601‐mhCMV‐ABEmaxNGA‐C3‐ E53ogRNA

Plasmid

#187063

Purpose

Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mhCMV

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MITF-M (G244R)

Plasmid

#31155

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Glycine 244 changed to Arginine

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
FN207

Plasmid

#50497

Depositor

Kenneth Yamada

Article

Danen et al J Biol Chem. 1995 Sep 15;270(37):21612-8

Insert

NM_001306129.1 (FN1 Human)

Expression

Bacterial

Mutation

repeats 6-10

Promoter

T7

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pmalc4x_I499F_TEV

Plasmid

#47703

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with I499F mutation (MYOC Human)

Tags

MBP

Expression

Bacterial

Mutation

changed I499F

Promoter

Ptac

Available Since

Sept. 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pIC389

Plasmid

#37387

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

LGN (GPSM2 Human)

Use

Retroviral

Tags

GFP, S-tag, and TEV

Expression

Mammalian

Mutation

deleted amino acid 1-364 and 524

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.MYB

Plasmid

#184660

Purpose

Retroviral expression of mouse RAP1 delta MYB

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta MYB

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.COIL

Plasmid

#184659

Purpose

Retroviral expression of mouse RAP1 delta COIL

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

pLPC

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta COIL

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.BRCT

Plasmid

#184658

Purpose

Retroviral expression of mouse Rap1 delta BRCT

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

RAP1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta BRCT

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.RCT

Plasmid

#184661

Purpose

Retroviral expression of mouse RAP1 delta RCT

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta RCT

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF1-ERBB4 (E836K)

Plasmid

#29542

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
MSCV-miRE_shBRD9_508-SV40-GFP

Plasmid

#75138

Purpose

Retroviral expression plasmid encoding shBRD9_508 (targeting human BRD)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_508

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
LifeAct-TagBFP-DiB-RM

Plasmid

#168782

Purpose

Actin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RM

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

LifeAct-TagBFP-DiB-RM

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRRG199-bmp4

Plasmid

#99076

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Bmp4 (also known as bmp)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Bmp4 (also known as bmp) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-WT Split GFP

Plasmid

#69891

Purpose

Expresses a GFP circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

GFP, laccase2 (stw Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Man1B1-Myc

Plasmid

#163645

Purpose

Expresses myc tagged Man1B1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mannosidase alpha class 1B member 1 (MAN1B1 Human)

Tags

myc

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMIEG3-NFAT2

Plasmid

#40351

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2006 Jan 15;176(2):880-8.

Insert

NFAT2 (NFATC1 Mouse)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only