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LI F-G neo c2

Plasmid

#54405

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

neomycin phosphotransferase II (nptII)

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro.3xBsmBI

Plasmid

#196709

Purpose

Backbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHnCB

Plasmid

#52064

Purpose

expresses nine genes from the H. neapolitanus carboxysome operon; IPTG-inducible

Depositor

David Savage

Article

Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

Insert

CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMM100

Plasmid

#127225

Purpose

AtU6::sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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TOPO 2-5

Plasmid

#68408

Purpose

TOPO backbone for GMAP genes with sites #2 and #5

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Type

Empty backbone

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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msEGFP-PACR_pRSETB

Plasmid

#55775

Purpose

Genetically encoded caged Ca2+

Depositor

Takeharu Nagai

Article

Fukuda et al ACS Chem Biol. 2014 May 16;9(5):1197-203

Insert

PACR

Tags

6xHis and msEGFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 3, 2014

Availability

Academic Institutions and Nonprofits only
pFH58

Plasmid

#128184

Purpose

Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRSETA-alphaFlincG

Plasmid

#19735

Depositor

Wolfgang Dostmann

Article

Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

alpha-FlincG

Use

Protein expression

Tags

His

Expression

Bacterial

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pWS1037

Plasmid

#123049

Purpose

G_ subunit

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 [3-4a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL30004

Plasmid

#50302

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, YFP variant of GFP (Aequorea victoria)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL80004

Plasmid

#50325

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pWS1837

Plasmid

#123058

Purpose

_-factor pheromone

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

_-factor [3-4a]

Expression

Yeast

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-11 (1wa3)

Plasmid

#80122

Purpose

Palm1-Late1-I3-01-myc (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-11 (1wa3)

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pAGT572_Nemo

Plasmid

#153487

Purpose

Level 1 episomal expression plasmid for S. cerevisiae

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIP-Rx3(A6)-mEGFP

Plasmid

#35530

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Rx3(A6)mEGFP

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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pRVdG-EGFP-N-P-M-SynPhRFP-L

Plasmid

#52479

Purpose

Expresses EGFP and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

EGFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pCF711

Plasmid

#121662

Purpose

U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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LI B-C mOrange

Plasmid

#54427

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

mOrange + 16 aa GS linker

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LII ins 2-3

Plasmid

#54379

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

insulator sequence, dummy 2-3

Use

Cloning vector

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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LII ins 4-5

Plasmid

#54380

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

insulator sequence, dummy 4-5

Use

Cloning vector

Available Since

July 24, 2014

Availability

Academic Institutions and Nonprofits only

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