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hHSF1 RNAi resistant

Plasmid

#118347

Purpose

This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

RNAi resistance mutations (does not change amino …

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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CLuc-FKBP

Plasmid

#31184

Depositor

David Piwnica-Worms

Article

Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

C-terminus of Firefly Luciferase and FK506-binding protein 12 (FKBP1A Human)

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB3m

Plasmid

#120289

Purpose

Produce human recombinant mTGFB3 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

monomeric Transforming Growth Factor-β3

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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Sumo3 PolQM1

Plasmid

#78462

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; Q2513R (please see depositor commen…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pLEGFP-Y705F-STAT3

Plasmid

#71445

Purpose

Retroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Mutation

Y705F

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pFB1.HMBP.PrS.MTF2

Plasmid

#125169

Purpose

Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

MTF2 (MTF2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-AKAR4

Plasmid

#181957

Purpose

Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

AKAP79-AKAR4 (AKAP5 Human)

Tags

AKAP79, Cerulean, HIS tag, and cpVenus(E172)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-M13-TevC-IRES-SP6-Cre

Plasmid

#171620

Purpose

M13-TevC with Cre

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

M13-TevC-IRES-SP6-Cre

Use

AAV and Synthetic Biology

Tags

6x His

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pC001 - huLshC2C2-MBP for bacterial expression

Plasmid

#79150

Purpose

Expresses human codon-optimized LshC2c2 for purification in E. coli

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

C2c2

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep152

Plasmid

#136825

Purpose

Mammalian expression of the centrosomal protein Cep152 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep152 (CEP152 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-WT

Plasmid

#122690

Purpose

Express His-tagged PKM2 (wild type) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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tetO-GATA4

Plasmid

#46030

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GATA4 (GATA4 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

R43Q, G50S and N197S

Promoter

tetO

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 Hepatitis C Virus NS3/NS4A

Plasmid

#61696

Purpose

inducible dual expression of NS3 protease along with its cofactor NS4A

Depositor

Michael Johnson

Article

Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

Inserts

NS4A
NS3

Tags

his

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pTriEx-PA-Rac1

Plasmid

#22024

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Avena sativa (oat), Human)

Tags

6X His

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-ALMS1

Plasmid

#136828

Purpose

Mammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-ALMS1 (ALMS1 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET42b-HF-BE3

Plasmid

#87438

Purpose

Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)

Tags

His6

Expression

Bacterial

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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Brg1 Del2 pET28a

Plasmid

#122116

Purpose

Expression Brg1 amino acids 1-837 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

6XHis and His-T7

Expression

Bacterial

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Brg1 Del3 pET28a

Plasmid

#122117

Purpose

Expression Brg1 amino acids 836-1446 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

His-T7

Expression

Bacterial

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pHPSE-P6

Plasmid

#185907

Purpose

Engineered Human Heparanase mutant for e.coli expression

Depositor

Colin Jackson

Article

Whitefield et al RSC Chem Biol. 2022 Feb 15;3(3):341-349.

Inserts

HPSE1 (HPSE Human)
HPSE1 (HPSE Human, Synthetic)

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSN840

Plasmid

#184832

Purpose

Expresses human KIF5A(∆exon27) and human KLC1 in insect cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Inserts

KIF5A (∆exon27) (KIF5A Human)
KLC1 (KLC1 Human)

Use

Cre/Lox

Tags

His-FLAG and mScarlet-2xStrepII

Expression

Insect

Mutation

∆exon27 form of human KIF5A

Promoter

p10 and polyhedrin

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCerulean-PA-Rac1

Plasmid

#22030

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Avena sativa (oat), Human)

Tags

6X His and mCerulean

Expression

Bacterial, Insect, and Mamm…

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-synaptoPAC-minWPRE

Plasmid

#153085

Purpose

Expresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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LIC1B_Caspase-LOVN7C-7

Plasmid

#104629

Purpose

bacterial expression of light-activated caspase-3 with N7C-7 linker variation

Depositor

Jim Wells

Article

Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Caspase-3 (CASP3 Human)

Tags

His and LOV2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMT-Hrp48-ADARcd-V5

Plasmid

#81172

Purpose

identification of cell-specific targets of RNA binding proteins

Depositor

Michael Rosbash

Article

McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

Insert

Hrp48-ADARcd fusion (Hrb27C Fly)

Expression

Insect

Promoter

Drosophila metallothionein promoter

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pet16b-Avi-ubiquitin_rbs_BirA

Plasmid

#134897

Purpose

Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Inserts

Ubiquitin (UBB Human)
BirA

Tags

10HIS-AviTag-PreScission(3C)protease

Expression

Bacterial

Promoter

RBS and T7

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-ALMS1

Plasmid

#136877

Purpose

Mammalian expression of the centrosomal protein ALMS1 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-ALMS1 (ALMS1 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTorPE-R-GECO1

Plasmid

#32465

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

R-GECO1.0

Use

Lab constructed

Tags

6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

Substitutions relative to the mApple-derived anal…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.PHF1

Plasmid

#125167

Purpose

Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

PHF1 (PHF1 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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