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CTA2-mNG211

Plasmid

#141167

Purpose

Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

SUMO-CTA2-mNG211

Tags

SUMO

Expression

Bacterial

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-SPW

Plasmid

#190628

Purpose

Bacterial expression of genetically encoded protease indicator SPW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SPW

Expression

Bacterial

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf7

Plasmid

#163597

Purpose

Expresses GDF-7 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf7 (Gdf7 Mouse)

Expression

Mammalian

Mutation

Some Guanin and Cytosine were substituted to redu…

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CTTN

Plasmid

#67100

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CTTN (CTTN Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-PML peptide

Plasmid

#175246

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

PML (PML Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJMP1273

Plasmid

#119265

Purpose

E. coli attTn7 site with B. subtilis flanking sequence cloned into pACYC

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

E. coli attTn7

Expression

Bacterial

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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p15aC-3D1D-5

Plasmid

#121050

Purpose

Bacterial expression of PIS, PI4K and PI3P5K mutant E545K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 3-kinase catalytic subunit p110α of class IA E545K

Tags

myc and none

Expression

Bacterial

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_Mito

Plasmid

#65670

Purpose

Expresses Mito-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

cytochrome c oxidase subunit VIII

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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GST eIF2beta

Plasmid

#45900

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

eIF2S2 (Eif2s2 Mouse)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CSNK2A1

Plasmid

#67085

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CSNK2A1 (CSNK2A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-RAP1

Plasmid

#84525

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

no stop codon

Promoter

none

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSV HA eIF2beta WT

Plasmid

#45898

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Eif2s2 (Eif2s2 Mouse)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pMT1100

Plasmid

#8618

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

ribonuclease Sa3, barstar

Tags

phoA signal sequence to RNase Sa3

Expression

Bacterial

Mutation

In addition to the substitution of the barnase ge…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
p230-pOsAct1::hpt-pZmUbi::BdPetC

Plasmid

#129649

Purpose

Rieske FeS overexpression

Depositor

Susanne von Caemmerer

Article

Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

Insert

CDS of PetC gene encoding for the Rieske FeS subunit

Expression

Plant

Mutation

Codon modified for Golden Gate

Promoter

pZmUbi

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pFP122

Plasmid

#21669

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
PFA0445w-bio-His

Plasmid

#50811

Purpose

Expresses enzymatically monobiotinylated full length PFA0445w

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFA0445w

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PF10_0166-bio-His

Plasmid

#50801

Purpose

Expresses enzymatically monobiotinylated full length PF10_0166

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PF10_0166

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_Mito

Plasmid

#65669

Purpose

Expresses Mito-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

cytochrome c oxidase subunit VIII

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K651R

Plasmid

#84528

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K651R

Promoter

none

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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FCIV1-GR SS287AA/S155A

Plasmid

#72700

Purpose

Lentiviral expression of GR-2A phosphodeficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Lambert et al Mol Cell Biol. 2013 Sep;33(18):3700-14.

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

SS287AA/S155A

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only