163,570 results

1. SpCas9-NG

   Plasmid

   #138566

   Purpose

   Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-hSyn-EGFP

   Plasmid

   #114215

   Purpose

   Lentiviral vector (control) that targets expression of EGFP to neurons

   Depositor

   Xue Han

   Article

   Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   human synapsin (hSyn)

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-FGFR2c-V5/HIS

   Plasmid

   #201107

   Purpose

   expression of human FGFR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.18 CMV-Luc

   Plasmid

   #100984

   Purpose

   CMV luciferase reporter - firefly luciferase driven by CMV promoter

   Depositor

   Lee Helman

   Article

   Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

   Insert

   CMV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCKmatBC

   Plasmid

   #138587

   Purpose

   matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

   Depositor

   Markus Jeschek

   Article

   Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

   Insert

   bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   matB contains native GUG start codon

   Promoter

   Plac promoter

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWPI-N1ICD

   Plasmid

   #185525

   Purpose

   Constitutive overexpression of human NOTCH1 intracellular domain

   Depositor

   Eric Shusta, Sean Palecek

   Article

   Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

   Insert

   NOTCH1 intracellular domain (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mGreenLantern

   Plasmid

   #197928

   Purpose

   Monomer expression vector

   Depositor

   Salvatore Chiantia

   Article

   Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

   Insert

   mGreenLantern
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PRDX1A-c001

   Plasmid

   #175473

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.

   Depositor

   Opher Gileadi

   Insert

   PRDX1 (PRDX1 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCF142_U6-sgRNA

   Plasmid

   #225960

   Purpose

   U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   U6-sgRNA (recipient)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE-tdTomato

    Plasmid

    #50798

    Purpose

    Expression of tdTomato under the control of the TRE-promoter

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    TREtight driven tdTomato fluorescent protein
    

    Use

    Mutli-species

    Tags

    Expression

    Mutation

    Promoter

    TREtight

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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