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Showing: 1141 - 1170 of 1192 results

  4. pUC57-mini v2-HF24_F5

    Plasmid
    #195719
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#5 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195719-plasmid-map-sequence-id-387872

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  20. pNHT7::UMSBP::sNluc2_ORF2

    Plasmid
    #186766
    Purpose
    An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..
    Depositor
    Bo Wang
    Article
    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002
    Insert
    Nanoluciferase
    Use
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186766-plasmid-map-sequence-id-375146

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  23. Split 'n coexpress CUL3-RBX1

    Plasmid
    #52293
    Purpose
    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
    Depositor
    Brenda Schulman
    Article
    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1
    Inserts
    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    Use
    Tags
    GST, IRES, and TEV
    Expression
    Bacterial
    Mutation
    contains aa 1-309, contains aa 310-768, and conta…
    Promoter
    IRES and tac
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid
    #204215
    Purpose
    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.
    Depositor
    Guei-Sheung Liu
    Article
    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121
    Insert
    Cas13bt3 and U6.SapI sgRNA cloning site
    Use
    AAV and CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Codon optimisation by GenScript tool
    Promoter
    CMV/U6
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    204215-plasmid-map-sequence-id-455913

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  30. pNIA-CEN-mVenus-FLAG-LANS1-Set2

    Plasmid
    #122003
    Purpose
    mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast
    Depositor
    Brian Kuhlman
    Article
    Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d
    Insert
    mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    mVenus-FLAG
    Expression
    Yeast
    Mutation
    Set2 point mutations K538G, K539S, R549G, K550S, …
    Promoter
    ADH1
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    122003-plasmid-map-sequence-id-238611

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Showing: 1141 - 1170 of 1192 results