We narrowed to 7,456 results for: Lif

1. pBEL2079

   Plasmid

   #155159

   Purpose

   Expresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Mutation

   MlaF_L265A

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBEL1244

   Plasmid

   #155138

   Purpose

   Expresses MlaFED operon in E. coli

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   mlaFE-[6xHis-2xQH-TEV-mlaD]-mlaC operon
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   araBAD

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. FoxFTVC+bpFOG_Cdkn1b.a

   Plasmid

   #107523

   Purpose

   Negative regulator of cell cycle progression by G1/S transition inhibition

   Depositor

   Lionel Christiaen

   Article

   Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

   Insert

   Cdkn1b.a
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FoxFTVC+bpFOG_Cdkn1b.b

   Plasmid

   #107524

   Purpose

   Negative regulator of cell cycle progression by G1/S transition inhibition

   Depositor

   Lionel Christiaen

   Article

   Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

   Insert

   Cdkn1b.b
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDC24

   Plasmid

   #131368

   Purpose

   expression of MEG-3Cterm (aa545-862) in C. elegans

   Depositor

   Geraldine Seydoux

   Article

   Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

   Insert

   meg-3 (aa545-862) (meg-3 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDC21

   Plasmid

   #131367

   Purpose

   expression of MEG-3IDR (aa1-544) in C. elegans

   Depositor

   Geraldine Seydoux

   Article

   Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

   Insert

   meg-3 (aa1-544) (meg-3 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-MBP1-K295TAG-W340A.pcDNA3-k

   Plasmid

   #126627

   Purpose

   Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site.

   Depositor

   Sharona Gordon

   Article

   Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

   Insert

   Maltose Binding Protein
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Amino acid mutations: K295 to Amber codon and W34…

   Promoter

   CMV

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Nod-BFP2-TSERex

   Plasmid

   #124785

   Purpose

   Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

   Depositor

   Yulong Li

   Article

   Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

   Insert

   Nod-BFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNP159

   Plasmid

   #106361

   Purpose

   To insert a single copy of a construct at a site on Chr II, corresponding to GFP-labelled RAB-11 expressed specifically in the epidermis.

   Depositor

   Jonathan Ewbank

   Article

   Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

   Insert

   ttTi5605_SEC_dpy-7p_GFP_RAB-11_3'unc-54
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. P0050::rpaAD53A

    Plasmid

    #101831

    Purpose

    drives expression of rpaA D53A variant from an rpaA-independent promoter

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    RpaA D53A with p0050 promoter (+ gentamicin resistance casette)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    D53 mutated to A

    Promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a_Msm_RbpA

    Plasmid

    #87928

    Purpose

    Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes

    Depositor

    Seth Darst

    Article

    Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 1

    Insert

    RbpA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7P

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFLIP34

    Plasmid

    #65501

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-t7TFPt9-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFLIP35

    Plasmid

    #65502

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-TFPt9-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFLIP36

    Plasmid

    #65504

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-Cer-cMyc

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFLIP42

    Plasmid

    #65617

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-Cit-attR1 and attR2-mCer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. p218 pCMV-CREM-L

    Plasmid

    #8396

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM-L
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre, single LOX 511 site

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

17. C077

    Plasmid

    #174346

    Purpose

    Template for making mRNA for overexpressing frog PABPC1 (C-FLAG) in frog oocytes

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (pabpc1.S Frog)
    

    Use

    In vitro transcription template

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. C078

    Plasmid

    #174347

    Purpose

    Template for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytes

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    ePAB
    

    Use

    In vitro transcription template

    Tags

    FLAG

    Expression

    Mutation

    13 silent mutations: G243A, A273G, G339A, C345G, …

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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19. C233

    Plasmid

    #174349

    Purpose

    Template for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytes

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (M161A) (pabpc1.S Frog)
    

    Use

    In vitro transcription template

    Tags

    FLAG

    Expression

    Mutation

    M161A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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20. PiggyBac dCas9-KRAB

    Plasmid

    #191314

    Purpose

    piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    KRAB-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1_AGIA-AirID

    Plasmid

    #182210

    Purpose

    Express AirID in mammalian cells.

    Depositor

    Shogo Nakano, Akira Nozawa, Sohei Ito, Tatsuya Sawasaki

    Article

    Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

    Insert

    AGIA-AirID
    

    Use

    Tags

    AGIA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 4xmts-G-Ca-FLITS

    Plasmid

    #191462

    Purpose

    Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    4xmts

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

    Plasmid

    #202569

    Purpose

    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    LINE-1
    

    Use

    Tags

    GFP-AI retrotransposition reporter and HaloTag7 o…

    Expression

    Mammalian

    Mutation

    Changed ORF1p Arginine 261 to Alanine

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pH2B-mNG2(11)

    Plasmid

    #206043

    Purpose

    Plasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.

    Depositor

    Alisa Piekny

    Article

    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

    Insert

    H2B gene fused to the mNG2(11) fragment
    

    Use

    Synthetic Biology

    Tags

    H2B-mNG2(11)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167937

    Purpose

    Inducible CRISPRa expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    dxCas9(3.7)-VPR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    tetON

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFsynW SYP-GFP

    Plasmid

    #197283

    Purpose

    Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYP and GFP (Syp Rat)
    

    Use

    Lentiviral

    Tags

    1D4 epitope

    Expression

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTP264

    Plasmid

    #149334

    Purpose

    Bacterial expression of Biotin ligase BirA

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Biotin ligase BirA
    

    Use

    Tags

    His14 and bdNEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-TK-Firefly-Control

    Plasmid

    #122277

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Thymidine kinase

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTAMAHISTEV_BauA

    Plasmid

    #128952

    Purpose

    expresses BauA in E.coli

    Depositor

    James Naismith

    Article

    Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 1

    Insert

    ferric acinetobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-66)

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. P307-FBP_6

    Plasmid

    #139697

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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