We narrowed to 5,797 results for: ATC

1. pCS2+-hsAgo2

   Plasmid

   #221718

   Purpose

   Expression of human Ago2 in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   hsAgo2 (AGO2 Human)
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   Promoter

   simian CMV IE94

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM-U6-reRNA-mmSCN9A

   Plasmid

   #205636

   Purpose

   reRNA guide targeting Mus Musculus SCN9A

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-mmSCN9A (Scn9a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. USP7 shRNA-TRE

   Plasmid

   #225338

   Purpose

   shRNAmir backbone under tet-operator for RNA interference, with YFP

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   USP7 shRNA (Usp7 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (tetracycline-responsive element)

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiCRISPRv2 EGFP sgRNA4

   Plasmid

   #164934

   Purpose

   Expresses EGFP sgRNA4 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LentiCRISPRv2 NT sgRNA5

   Plasmid

   #164930

   Purpose

   Expresses Non-targeting sgRNA5 control in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   Non-targeting sgRNA5 (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-VcnTS-E1015A

   Plasmid

   #213415

   Purpose

   Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnTS-E1015A (VCL Synthetic, Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1015 to alanine (E…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-VcnTS-E1021A

   Plasmid

   #213416

   Purpose

   Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnTS-E1021A (VCL Synthetic, Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1021 to alanine (E…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-VcnCS-E1015A

   Plasmid

   #213412

   Purpose

   Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnCS-E1015A (VCL Synthetic, Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1015 to alanine (E…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-VcnCS-E1021A

   Plasmid

   #213413

   Purpose

   Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnCS-E1021A (VCL Synthetic, Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1021 to alanine (E…

   Promoter

   CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFP-ST-P2A-pSC-CAAX

    Plasmid

    #207637

    Purpose

    A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    CMV

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXPR_003 sgRELA guide 2

    Plasmid

    #193592

    Purpose

    RELA knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgRELA guide 2 (RELA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV.CBA.YFP.miR-E_shSrpk3-2

    Plasmid

    #180401

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-2 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mU6-sgCebpa_v2-hu6-sgCebpb_v2

    Plasmid

    #177252

    Purpose

    Expresses Cebpa_v2 (mU6), Cebpb_v2(hU6) gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgCebpa_v2/sgCebpb_v2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6/hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mU6-sgCebpa_v2-hu6-sgCebpd_v2

    Plasmid

    #177254

    Purpose

    Expresses Cebpa_v2 (mU6), Cebpd_v2(hU6) gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgCebpa_v2/sgCebpd_v2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6/hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hu6-sgCebpa_v2 (opti)

    Plasmid

    #177246

    Purpose

    Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpa gRNA

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgCebpa_2nd
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMBP-HRV3C-AcrIF9

    Plasmid

    #141442

    Purpose

    Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

    Depositor

    Blake Wiedenheft

    Article

    Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

    Insert

    AcrIF9
    

    Use

    Tags

    HRV3C cleavage site and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px335-sgRNA-EF(Cas9n)-Ctcf-R

    Plasmid

    #122320

    Purpose

    Expresses sgRNA targeting mouse Ctcf and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Ctcf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-h-mmPtch1-B-V111FL114FI766F-HA-TagBFP

    Plasmid

    #120911

    Purpose

    Expresses BFP tagged Ptch1-B (V111FL114FI766F)

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291, Y…

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFP

    Plasmid

    #120908

    Purpose

    Expresses Ptch1-B (V111FL114FW115A)

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-h-mmPtch1-B-I766FV111F-HA-GFP

    Plasmid

    #120907

    Purpose

    Expresses Ptch1-B (I766FV111F)

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA-h-mmPtch1-B-Y233C-HA

    Plasmid

    #120904

    Purpose

    Expresses Ptch1-B (Y233C) for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291, Y…

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA-h-mmPtch1-B-L234C-HA

    Plasmid

    #120905

    Purpose

    Expresses Ptch1-B (L234C)

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291, L…

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NSL1 C6.4 gRNA

    Plasmid

    #90805

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NSL1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NSL1 (Guide Designation C6.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CCT5 B8.4 gRNA

    Plasmid

    #90583

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CCT5

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CCT5 (Guide Designation B8.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. COPS3 G11.4 gRNA

    Plasmid

    #90638

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human COPS3

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    COPS3 (Guide Designation G11.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TPX2 H1.2 gRNA

    Plasmid

    #90918

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TPX2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    TPX2 (Guide Designation H1.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CDk4-luciferase fragment B

    Plasmid

    #86659

    Purpose

    Luciferase reporter containing CDK4 promoter

    Depositor

    Jaime Modiano

    Article

    Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

    Insert

    CDK4 promoter (CDK4 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    generated by cleavage at the KpnI site from 880 (…

    Promoter

    CDK7

    Available since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AP56-6

    Plasmid

    #65593

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APa13
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

29. AP56-5

    Plasmid

    #65591

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APa13
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

30. AP56-8

    Plasmid

    #65596

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APa13
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

31. AP56-7

    Plasmid

    #65594

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APa13
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pVRa34_3302

    Plasmid

    #49694

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF34_3302
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti-TripleColour-StAR

    Plasmid

    #222695

    Purpose

    Competition assay

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP, miRFP703, dTomato

    Expression

    Mammalian

    Mutation

    PGK, TagBFP, dTomato without BsmBI cutsite

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. TurboID-EV

    Plasmid

    #215085

    Purpose

    Expresses TurboID in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    TurboID (birA )
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSpCas9(BB)-2A-tomato SOX2-sgRNA

    Plasmid

    #196190

    Purpose

    Editing human SOX2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    SOX2 (SOX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

    Plasmid

    #71396

    Purpose

    2Gi2R assay

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Inserts

    2xGFP:NES
    2xRFP:NLS
    

    Use

    Lentiviral

    Tags

    IRES

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha and IRES

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEM5Z(+)-EGFP

    Plasmid

    #65206

    Purpose

    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.

    Depositor

    LuZhe Sun

    Article

    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Restriction enzyme recognition sites added either…

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pLVX-EF1a-tetOn-IRES-G418 (EtO)

    Plasmid

    #84776

    Purpose

    Second generation lenti tetracycline operator (on)

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Insert

    TET OPERATOR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pTIGHT

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. KillerRed-LacI-NLS

    Plasmid

    #215102

    Purpose

    Expresses KillerRed-LacI-NLS in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    KillerRed-LacI-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HA-human-BNIP3

    Plasmid

    #100781

    Purpose

    Mammalian expression of BNIP3

    Depositor

    Joseph Gordon

    Article

    Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

    Insert

    human-BNIP3 (BNIP3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge