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pUC19-P70a-Top-T~Bottom-T

Plasmid

#87315

Purpose

Control plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system (promoter control)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60 delta gap

Plasmid

#49091

Purpose

Template for generating gene 60 mRNA, coding gap deletion

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Use

Tags

Expression

Mutation

Coding gap and coding gap STOP have been deleted.

Promoter

T7

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Gm-LacZ30

Plasmid

#65028

Purpose

GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 3. See Depositor Comments below for information regarding the lacZ sequence.

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Type

Empty backbone

Use

Tags

lacZ

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUC 3GLA UAS HAi

Plasmid

#71763

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(1004)

Plasmid

#44366

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(1004) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(1004)

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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Mishler2014-No-aptamer-controlluciferase-pUC19

Plasmid

#63856

Purpose

This pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

No-aptamer control mRNA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

tac

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B8-TEF(203)

Plasmid

#44378

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(203) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(203)

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(804)

Plasmid

#44368

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(804) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(804)

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19_msfGFP-HP1a-repair-template

Plasmid

#238248

Purpose

For knock-in msfGFP to HP1a locus

Depositor

Denes Hnisz

Insert

HP1a

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-SRRM2-repair-template

Plasmid

#238247

Purpose

For knock-in msfGFP to SRRM2 locus

Depositor

Denes Hnisz

Insert

SRRM2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-EWSR1-repair-tempate

Plasmid

#237682

Purpose

For knock-in msfGFP to EWSR1 locus

Depositor

Denes Hnisz

Insert

EWSR1 (EWSR1 Human)

Use

CRISPR

Tags

msfGFP

Expression

Mutation

Promoter

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-TCOF1-repair-template

Plasmid

#237632

Purpose

For knock-in msfGFP to TCOF1 locus

Depositor

Denes Hnisz

Insert

TCOF1 (TCOF1 Human)

Use

CRISPR

Tags

msfGFP

Expression

Mutation

Promoter

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-FUS-repair-tempate

Plasmid

#237684

Purpose

For knock-in msfGFP to FUS locus

Depositor

Denes Hnisz

Insert

FUS (FUS Human)

Use

CRISPR

Tags

msfGFP

Expression

Mutation

Promoter

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca+C-Tail

Plasmid

#237514

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca+C-Tail

Use

Tags

Expression

Plant

Mutation

Add C-terminal extension

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

Plasmid

#228253

Purpose

pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

mpknot entry plasmid backbone for epegRNA expression via human U6 promoter

Use

Tags

Expression

Mammalian

Mutation

n/a

Promoter

U6

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUCmini-RELA-PGK-EGFP

Plasmid

#228051

Purpose

Homologous donor template for RELA knockout expressing EGFP

Depositor

Yohei Sato

Article

Sato et al Clinical Immunology Communications, Volu

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-RELB-PGK-EGFP

Plasmid

#228052

Purpose

Homologous donor template for RELB knockout expressing EGFP

Depositor

Yohei Sato

Article

Sato et al Clinical Immunology Communications, Volu

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-RELC-PGK-EGFP

Plasmid

#228053

Purpose

Homologous donor template for RELC knockout expressing EGFP

Depositor

Yohei Sato

Article

Sato et al Clinical Immunology Communications, Volu

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-RELA-PGK-tdTomato

Plasmid

#228054

Purpose

Homologous donor template for RELA knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Clinical Immunology Communications, Volu

Insert

tdTomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-RELC-PGK-tdTomato

Plasmid

#228056

Purpose

Homologous donor template for RELC knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Clinical Immunology Communications, Volu

Insert

tdTomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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