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MCV-hVam6p-

Plasmid

#32059

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-hVam6p-

Mutation

TG to GC mutations at 3280-3281 (nt) of full cons…

Promoter

none from vector. Insert contains native promoter…

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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p6721 MSCV-IP N FlagHA 16E6 8S9A10T

Plasmid

#44153

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

E6 8S9A10T mutant

Promoter

MSCV LTR

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
Syn3E34A

Plasmid

#13401

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

Myc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector. E34 of s…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
3445 pcDNA3 Bid p15

Plasmid

#8812

Depositor

Stanley Korsmeyer

Article

Zha et al Science 2000 Dec 1;290(5497):1761-5.

Insert

Bid p15 (Bid Mouse)

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
p6722 MSCV-IP N FlagHA 16E6 I128T

Plasmid

#44154

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

HPV16 E6 I128T mutant

Promoter

MSCV LTR

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
pAM5187

Plasmid

#120086

Purpose

Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid.

Depositor

Susan Golden

Article

Chen et al Microbiology. 2016 Dec;162(12):2029-2041

Type

Empty backbone

Use

Shuttle vector

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCDC86-Halo

Plasmid

#175323

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC86 (CCDC86 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-MRPS2

Plasmid

#31342

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein S2 mature form (MRPS2 Human)

Tags

His

Expression

Bacterial

Mutation

Encodes the predicted mature form for incorporati…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pCGN-ELK-1 R mut

Plasmid

#27159

Purpose

For mammalian expression of human Elk-1 with an HA tag and a mutation in the R repression domain

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Mutation

R box mutant: K249A/V250A/E251A

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only
TOPO CMV

Plasmid

#68454

Purpose

TOPO construct expressing CMV for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cytomegalovirus Immediate-Early Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBS YBX1

Plasmid

#19217

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

YBX1 (YBX1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pPV454

Plasmid

#42922

Depositor

James Lok

Article

Stoltzfus et al PLoS One. 2012;7(6):e38587. doi: 10.1371

Insert

Ce-age-1 promoter (873 bp)::Ce-age-1 cDNA (3549 bp)::Ce-unc-54 terminator (765 bp) (age-1 Nematode)

Expression

Worm

Promoter

Ce-age-1 promoter (873 bp)

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pIS47 C20orf139 3'UTR

Plasmid

#14489

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

C20orf139 3'UTR (SRXN1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pX459V2.0+K848A

Plasmid

#108296

Purpose

pX459 V2.0 (Plasmid #62988) with the K848A mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLX302-NIPP1-V5 puro

Plasmid

#87775

Purpose

Expresses V5 tagged human NIPP1

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

NIPP1 (PPP1R8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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Or45b-Gal4

Plasmid

#63036

Purpose

To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or45b, using the promoter sequences of the gene cloned 5' of Gal4

Depositor

Leslie Vosshall

Article

Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

Insert

Drosophila melanogaster Or45b promoter

Tags

Drosophila ADH (aa's 1-18) fused to Yeast Ga…

Expression

Insect

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-ABEmax(TadA E59A, TadA*E59A)

Plasmid

#125649

Purpose

pCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* E59A)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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SERA7-bio-His

Plasmid

#50819

Purpose

Expresses enzymatically monobiotinylated full length SERA7

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA7

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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BC-A1-014

Plasmid

#78702

Purpose

A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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MTK3a_001

Plasmid

#123754

Purpose

Encodes plasma membrane localization signal from Lyn kinase as a Type 3a part to be used in the MTK

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LYN

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-SNAP-HCA_sensor

Plasmid

#127181

Purpose

Bacterial expression of the fusion protein used for sulfa-drugs sensing

Depositor

Kai Johnsson

Article

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA

Tags

HisTag and StrepTag

Expression

Bacterial

Promoter

T7

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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AU1-TTC30A1-pcDNA3

Plasmid

#47323

Purpose

Expresses AU1 epitope-tagged TTC30A1 in mammalian cells

Depositor

Richard Maurer

Article

Howard et al Exp Cell Res. 2013 Jun 25. pii: S0014-48

Insert

TTC30A1 (Ttc30a1 Mouse)

Tags

AU1 epitope

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pOVC1_RsLP-LOV_X

Plasmid

#131177

Purpose

Expresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

RsLP-LOV

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pJOG331

Plasmid

#105408

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

6xHA tag fused to Twin-Strep tag

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPAG1_TPR

Plasmid

#109873

Purpose

Protein expression and purification of SPAG1_TPR

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SPAG1_TPR (SPAG1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MTK4a_014

Plasmid

#123827

Purpose

Encodes a nuclear localized mAzamiGreen coexpression cassette as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

P2A::NLS::mAzamiGreen

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-nirButterfly

Plasmid

#136590

Purpose

Expression of a genetically-encoded voltage indicator nirButterfly

Depositor

Vladislav Verkhusha

Article

Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

Insert

nirButterfly

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pNP1 OR sfGFP

Plasmid

#107364

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-CHC1

Plasmid

#18850

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

CHC1 (CHC1 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-DJ1-M26I

Plasmid

#29353

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Met26Ile

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only