We narrowed to 26,489 results for: Vit;

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pET21a-C2-mKate

Plasmid

#208763

Purpose

To express C2-mKate in bacteria

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2-mKate (Mfge8 Mouse)

Tags

mKate

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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ABCA1 promoter luciferase reporter (Full-length)

Plasmid

#86442

Purpose

Human ABCA1 promoter luciferase reporter (Full-length)

Depositor

David Mu

Article

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Insert

ABCA1 promoter (ABCA1 Human)

Use

Luciferase

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV CaMKIIa iChloC 2A tDimer

Plasmid

#66709

Purpose

Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

Depositor

Thomas Oertner

Article

Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Expression

Mammalian

Mutation

E83Q,E90R,E101S,D156N,T159C

Promoter

CaMKIIa

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMRX-hSTING (S366A)

Plasmid

#214163

Purpose

Retroviral transduction of STING

Depositor

Nan Yan

Article

Wu et al Immunity. 2020 Jul 14;53(1):115-126.e5.

Insert

STING (S366A) (STING1 Human)

Use

Retroviral

Mutation

S366A

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-hSTING (R232)

Plasmid

#214162

Purpose

Retroviral transduction of STING

Depositor

Nan Yan

Article

Wu et al Immunity. 2020 Jul 14;53(1):115-126.e5.

Insert

STING (STING1 Human)

Use

Retroviral

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pICE-HA-MRE11-H63S

Plasmid

#82035

Purpose

Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

MRE11 (MRE11 Human)

Tags

HA

Expression

Mammalian

Mutation

H63S MRE11 mutant

Promoter

CMV-tet

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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mCardinal-ZO1-C-14

Plasmid

#56179

Purpose

Localization: Tight Junctions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

ZO1 (TJP1 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pJJGL001

Plasmid

#180605

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

DpbCasX

Use

CRISPR

Tags

10x His and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomArchon

Plasmid

#126941

Purpose

Expression of SomArchon under Syn promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Synapsin

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-hUba2-His

Plasmid

#53117

Purpose

expression of full length human Uba2 with Cterminal His-tag in E.coli

Depositor

Frauke Melchior

Article

Werner et al Methods Mol Biol. 2009;497:187-99. doi:

Insert

UBA2 (UBA2 Human)

Tags

His and T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP gRNA (BRDN0000561167)

Plasmid

#80034

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGEXtev-HMG54kd

Plasmid

#26030

Depositor

Johann Deisenhofer

Article

Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

Insert

3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)

Tags

GST

Expression

Bacterial

Mutation

Catalytic domain (amino acids 426-488) Methion…

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pWPXL-Sox4

Plasmid

#36984

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox4 (SOX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 13, 2012

Availability

Academic Institutions and Nonprofits only

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Myc-Bnip3FL

Plasmid

#100796

Purpose

Mammalian expression of Bnip3 full length splice variant

Depositor

Joseph Gordon

Article

Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

Insert

mus Bnip3 (Bnip3 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pRN3P-NLS-EGFP-deadBtgh-3NLS

Plasmid

#194470

Purpose

Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

Depositor

Mathieu Boulard

Article

Formichetti et al Dev Cell 10 Jan 2024

Insert

BtGH84

Use

For in vitro transcription from t3 promoter after…

Tags

SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

Mutation

Aspartic Acid 242 (in Dennis et al.) changed to A…

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-AktAR2

Plasmid

#184052

Purpose

Lysosome-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Lyso-ExRai-AktAR2

Tags

Lysosome-associated membrane protein 1 (LAMP1).

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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MRGPRD-DuET

Plasmid

#213340

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

MRGPRD (MRGPRD Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-p38alpha

Plasmid

#47575

Purpose

Bacterial expression plasmid for GST-3xFlag-tagged p38alpha

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

p38alpha (Mapk14 Mouse)

Tags

3xFlag and GST

Expression

Bacterial

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTriEx-RhoA FLARE.sc Biosensor Q63L

Plasmid

#12151

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor Q63L (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA Q63L (dominant-positive mutation)

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-hST3GAL6-RFP

Plasmid

#22923

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human type 2 lactosamine alpha-2,3-sialyltransferase promoter driving DsRedExpress (ST3GAL6 Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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