161,261 results

1. TFORF2091

   Plasmid

   #144382

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FANCD2 (FANCD2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

   Plasmid

   #48285

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a_T7-ARG2

   Plasmid

   #106474

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBITE-EGFRvIII-F269-OKT3

   Plasmid

   #190681

   Purpose

   Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT7TS-rich

   Plasmid

   #99050

   Purpose

   This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.

   Depositor

   Richard Bélanger

   Type

   Empty backbone

   Use

   Unspecified; Xenopus oocyte system

   Tags

   Expression

   Mutation

   Promoter

   pT7

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-Syn-PSD95-mCherry

   Plasmid

   #125694

   Purpose

   Exogenous expression of PSD95 (red fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLSC-5

   Plasmid

   #62889

   Purpose

   Lenti vector for expression of inducible split Cas9

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Inserts

   SpCas9(574-1368)
   SpCas9(2-573)
   

   Use

   CRISPR and Lentiviral

   Tags

   FFBP12, FRB, NES PTK2, NLS SV40, and P2A

   Expression

   Mammalian

   Mutation

   *see comment below

   Promoter

   EFS

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-N-Flag-NLRP3 711-1034

   Plasmid

   #75141

   Purpose

   Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 711-1034

   Promoter

   CMV

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.0-Magneto2.0-p2A-mCherry

   Plasmid

   #74308

   Purpose

   Bicistronic expression of Magneto2.0 and mCherry in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   Magneto2.0 (FTL, FTH1, Trpv4 Rat, Human)
   

   Use

   Tags

   Flag and p2A-mCherry

   Expression

   Mammalian

   Mutation

   TRPV4: delta 760–871

   Promoter

   CMV

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CRISPR_HLA_class_I_1

    Plasmid

    #164986

    Purpose

    Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA class I
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-TBG-ER-TurboID

    Plasmid

    #149415

    Purpose

    Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    ER-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    TBG

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHA-eIF4E

    Plasmid

    #17343

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    eIF4E (EIF4E Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT3TS-nCas9n

    Plasmid

    #46757

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T3

    Available since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HP138-neo

    Plasmid

    #134247

    Purpose

    Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    Contains Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5/FRT/TO GFP

    Plasmid

    #19444

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDNA2.0-6H-TEV-KRAS G12D

    Plasmid

    #159439

    Purpose

    Expresses KRAS G12D in E.coli

    Depositor

    Kenneth Westover

    Article

    Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

    Insert

    KRAS G12D (KRAS Human)
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-N-Flag-NLRP3 1-90

    Plasmid

    #75137

    Purpose

    Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 1-90

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hSyn-somBiPOLES-mCerulean

    Plasmid

    #154945

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. m168

    Plasmid

    #34886

    Depositor

    Irvin Chen

    Article

    Morizono et al Nat Med. 2005 Mar;11(3):346-52. Epub 200

    Insert

    Sindbis virus envelope protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Syn-ChrimsonR-tdT

    Plasmid

    #59171

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge