Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 19,142 results for: Tec

Clear keywords
Showing: 7801 - 7820 of 19142 results

  6. B-HiFi SpCas9

    Plasmid
    #207362
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HiFi SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    R691A, amino acids 1005-1013 replaced with two gl…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207362-plasmid-map-sequence-id-410135

    Enlarge

  9. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

    Plasmid
    #176531
    Purpose
    PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.
    Depositor
    Jared Toettcher
    Article
    Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13
    Inserts
    KGV transcription factor
    TagBFP
    Use
    Piggybac transposase integration vector
    Tags
    ErkKTR, Gal4 DNA binding domain, PEST destabiliza…
    Expression
    Mammalian
    Available Since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    176531-plasmid-map-sequence-id-375608

    Enlarge

  10. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

    Plasmid
    #218509
    Purpose
    integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mKate2
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Deletion of Plac_mrfp cassette, introduction of a…
    Promoter
    P23
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218509-plasmid-map-sequence-id-438730

    Enlarge

  11. SpCas9-NG-ABE8e(V106W)

    Plasmid
    #198533
    Purpose
    Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198533-plasmid-map-sequence-id-388880

    Enlarge

  14. pGES201

    Plasmid
    #190197
    Purpose
    For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection
    Depositor
    Yuefeng Guan
    Article
    Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7
    Insert
    spCas9
    Use
    CRISPR
    Tags
    3xFlag
    Expression
    Plant
    Mutation
    plant-codon optimized
    Promoter
    Glycine max elongation factor 1A(pM4)
    Available Since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190197-plasmid-map-sequence-id-371938

    Enlarge

  18. pSL3718 (pQCascade_Pse)

    Plasmid
    #200899
    Purpose
    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.
    Depositor
    Samuel H. Sternberg
    Article
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Insert
    PseCas7, PseCas8, PseCas6, PseTniQ
    Tags
    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)
    Expression
    Mammalian
    Available Since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200899-plasmid-map-sequence-id-393105

    Enlarge

Showing: 7801 - 7820 of 19142 results