159,117 results

1. pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

   Plasmid

   #162278

   Purpose

   Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

   Depositor

   Al George

   Article

   DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

   Insert

   SCN1A Variant 1, stabilized (SCN1A Human)
   

   Use

   Tags

   IRES-mScarlet

   Expression

   Mammalian

   Mutation

   IVS intron inserted after bp2946. Silent codon c…

   Promoter

   CMV

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmTurquoise2-Golgi

   Plasmid

   #36205

   Purpose

   In vivo visualization of golgi apparatus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   Golgi targeting sequence (B4GALT1 Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE2-TetOminiCMV-SV40LT

   Plasmid

   #136616

   Purpose

   Dox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cells

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   SV40 large T antigen
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD33-mf-lon

   Plasmid

   #21867

   Depositor

   Robert Sauer

   Article

   Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

   Insert

   Lon (Mfl404 Mesoplasma florum)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   all TGA codons changed to TGG codons

   Promoter

   Available since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. human STIM1 YFP

   Plasmid

   #19754

   Depositor

   Anjana Rao

   Article

   Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Retroviral

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEGFP-C1

   Plasmid

   #54759

   Purpose

   Localization: C1 Cloning Vector, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLVX-TetOne-Puro-EcSTH-FLAG

   Plasmid

   #218628

   Purpose

   This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   EcSTH
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-BBS2

   Plasmid

   #218712

   Purpose

   Expresses N-terminally EGFP-tagged BBS2 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

   Insert

   BBS2 (BBS2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pExp-GB1

   Plasmid

   #112565

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-GB1 and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ubc.Luc.IRES.Puro

    Plasmid

    #33307

    Depositor

    Linzhao Cheng

    Article

    Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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