We narrowed to 13,692 results for: Egf

1. pCellFree_G03 GATA1

   Plasmid

   #67068

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GATA1 (GATA1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pmyc-GFP-TNRC6A-del.GW-I+II+III-NES-mut

   Plasmid

   #42040

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   deleted aa457-532, aa716-772 and aa804-924, F951A…

   Promoter

   CMV

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHJ424

   Plasmid

   #20682

   Depositor

   Stephen Shaw

   Article

   Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

   Insert

   Ezrin (EZR Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Lys 253, 254, 262, 263 to Asn

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

4. 1161F

   Plasmid

   #183138

   Purpose

   Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.

   Depositor

   Omar Akbari

   Article

   Kandul et al GEN Biotechnology (2022) 1:4, 372-385

   Insert

   U6.3-gRNAs[sxl, bTub]
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FUP95PDZGW (C5)

   Plasmid

   #74021

   Purpose

   lentiviral expression of Psd95-EGFP fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95 (DLG4 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   PDZ

   Promoter

   hUbC

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pGP-CMV-NEW-fRGECO2

   Plasmid

   #125248

   Purpose

   Expresses red fluorescent calcium sensor in mammalian cells

   Depositor

   Katalin Torok

   Article

   Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

   Insert

   fRGECO2
   

   Use

   Tags

   NES signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEAST-J23101-CocE

   Plasmid

   #114600

   Purpose

   "Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   CocE
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   J23101

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLL3.7 shRNA against CRTC3

   Plasmid

   #72712

   Purpose

   Lentiviral expression of shRNA against CRTC3 (3rd generation)

   Depositor

   Freddy Jeanneteau

   Article

   Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

   Insert

   CRTC3
   

   Use

   Lentiviral

   Tags

   CMV-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCellFree_G03 HDAC1

   Plasmid

   #67101

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pTK158_mCherry-4.1G-deltaCTD-hardened

    Plasmid

    #46360

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G (EPB41L2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Deleted amino acids 886-1005, and modified for si…

    Promoter

    CMV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMig (Martins Lab)

    Plasmid

    #14125

    Depositor

    L. Miguel Martins

    Article

    Nicke et al Mol Cell. 2005 Dec 9. 20(5):673-85.

    Insert

    IRES-EGFP
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Cla I sites were silenced in the original pRetroS…

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pMC-6-GFP-P2A-NptII-9

    Plasmid

    #197741

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    EGFP-P2A-NptII bicistronic CDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. iLID-mCherry-iTrkA

    Plasmid

    #136509

    Purpose

    Mammalian expression of iLID fused to mCherry and intracellular signaling domain of TrkA (aa 450-799)

    Depositor

    Bianxiao Cui

    Article

    Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

    Insert

    iLID mCherry iTrkA (Ntrk1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NLS-dCas9-HA-NLS-GFP4-VPR

    Plasmid

    #183924

    Purpose

    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    dCas9-GFP4-VPR
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Myc-CD4-mCherry-HA-FRB

    Plasmid

    #100740

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD4
    

    Use

    Tags

    FRB, HA, Myc, and mCherry

    Expression

    Mammalian

    Mutation

    Engineered to remove large part of extracellular …

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCellFree_G03 CTTN

    Plasmid

    #67100

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CTTN (CTTN Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pLL3.7 shRNA against CRTC1

    Plasmid

    #72710

    Purpose

    Lentiviral expression of shRNA against CRTC1 (3rd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    CRTC1
    

    Use

    Lentiviral

    Tags

    CMV-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCellFree_G03 NFKB1

    Plasmid

    #67126

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    NFKB1 (NFKB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pMIEG3-NFAT2

    Plasmid

    #40351

    Depositor

    Alexander Dent

    Article

    Wang et al J Immunol. 2006 Jan 15;176(2):880-8.

    Insert

    NFAT2 (NFATC1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

20. NKP33 GFP (1032)

    Plasmid

    #62022

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    C9orf46 (PLGRKT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pCellFree_G03 DDX5

    Plasmid

    #67111

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

22. N-term Hec1-targeted Aurora B FRET sensor

    Plasmid

    #45239

    Purpose

    sensor targeted to N-terminus of Hec1

    Depositor

    Michael Lampson

    Article

    Welburn et al Mol Cell. 2010 May 14;38(3):383-92. doi:

    Insert

    Hec1, Aurora B FRET sensor
    

    Use

    Tags

    Aurora B FRET sensor and Hec1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. sspa-GCaMP6f

    Plasmid

    #67561

    Purpose

    short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

24. px458_2A_GFP_sgRNA_PRKRA

    Plasmid

    #106109

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting PRKRA

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA targeting PRKRA (PRKRA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. spa-GCaMP6f

    Plasmid

    #67558

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    CMV

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pCellFree_G03 GTF2B

    Plasmid

    #67067

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2B (GTF2B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

27. ZJOM12

    Plasmid

    #133638

    Purpose

    Integration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pCUP1-GFP-Tlg2 (TLG2 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-CmTMC1truncated

    Plasmid

    #154908

    Purpose

    For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-CmTMC1truncated
    

    Use

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-243 and 843-969

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GFP-MuTMC2truncated

    Plasmid

    #154909

    Purpose

    For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-MuTMC2truncated
    

    Use

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-179 and 691-716

    Promoter

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCellFree_G03 IFNG

    Plasmid

    #67056

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IFNG (IFNG Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only