We narrowed to 11,714 results for: NSI

1. pGAL4-DBD-EOMES-Adel-IDR

   Plasmid

   #145230

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and EOMES-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   EOMES-Adel-IDR (EOMES Human)
   

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGAL4-DBD-HOXD13wt-IDR

   Plasmid

   #145244

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HOXD13wt IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13wt-IDR (HOXA13 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGAL4-DBD-GSX2-Adel-IDR

   Plasmid

   #145232

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and GSX2-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   GSX2-Adel-IDR (GSX2 Human)
   

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUG-T2A-F241S

   Plasmid

   #140740

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation F241S and GFP as two separate proteins

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-T2A-PTENF241S (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   F241S

   Promoter

   hUbiC

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUG-T2A-W274L

   Plasmid

   #140742

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation W274L and GFP as two separate proteins

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-T2A-PTENW274L (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   W274L

   Promoter

   hUbiC

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUG-T2A-D252G

   Plasmid

   #140741

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation D252G and GFP as two separate proteins

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-T2A-PTEND252G (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   D252G

   Promoter

   hUbiC

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST40-2XHA-Src-S4-UD-mCherr

   Plasmid

   #140307

   Purpose

   Expresses Flag-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   contains only the SH4 and UD regions fused to mCh…

   Promoter

   CMV

   Available Since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST40-2XFL-Src-S32

   Plasmid

   #140309

   Purpose

   Expresses Flag-tagged human Src-S32 in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   contains only the SH3 and SH2 domains

   Promoter

   CMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB139 - pL0_ZCT1 (CDS1)

   Plasmid

   #123184

   Purpose

   Golden Gate (MoClo; CDS1) compatible ZCT1 gene from Catharanthus roseus; a repressor of the STR1 promoter from C. roseus

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   ZCT1 from Catharanthus roseus
   

   Use

   Part for plant expression

   Expression

   Plant

   Mutation

   No BpiI and BsaI sites

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB142 - pL0_ORCA3 (CDS1)

    Plasmid

    #123185

    Purpose

    Golden Gate (MoClo; CDS1) compatible ORCA3 gene from Catharanthus roseus; an activator of the STR1 promoter from C. roseus

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    ORCA3 from Catharanthus roseus
    

    Use

    Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCRII apoBa

    Plasmid

    #121897

    Purpose

    Plasmid for making zebrafish apoBa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoBa (apoba Zebrafish)
    

    Use

    Unspecified

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

    Plasmid

    #122450

    Purpose

    Expression of inositol 1,4,5-trisphosphate 3-kinase A

    Depositor

    Christophe Erneux

    Article

    Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

    Insert

    inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
    

    Tags

    beta-galactosidase

    Expression

    Bacterial

    Mutation

    coding sequence from V66 till stop codon

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. eGFP-PRM-3R

    Plasmid

    #112156

    Purpose

    Mammalian eGFP expression plasmid for PRM-3R.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    PRM-3R (ABL1 Human)
    

    Expression

    Mammalian

    Mutation

    contains only the PRM domain of ABl1

    Promoter

    CMV

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMAL c2x Tral N

    Plasmid

    #113009

    Purpose

    For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    N terminus of tral (tral Fly)
    

    Expression

    Bacterial

    Mutation

    amino acids 1-355

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMAL c2x Tral FL-15A

    Plasmid

    #113011

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to A

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMAL c2x Tral C

    Plasmid

    #113008

    Purpose

    For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    C terminus of tral (tral Fly)
    

    Expression

    Bacterial

    Mutation

    amino acids 356-652

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMAL c2x Tral C 15A

    Plasmid

    #113007

    Purpose

    For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    C terminus of tral (tral Fly)
    

    Expression

    Bacterial

    Mutation

    PNG phosphorylation sites mutated to A

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. AF10_OM-LZ

    Plasmid

    #111753

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    MLLT10 (MLLT10 Human)
    

    Tags

    6x His - TEV

    Expression

    Bacterial

    Mutation

    Contains the OM-LZ region of AF10 (residues 720-7…

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. GFP-AuroraB L154A/H250Y

    Plasmid

    #108491

    Purpose

    expression of EGFP-AuroraB Analog sensitive (L154A/H250Y)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Analog sensitive L154A/H250Y

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_FRK-1/1

    Plasmid

    #91549

    Purpose

    Protein expression and purification of human SH3 domain construct FRK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FRK-1/1 (FRK Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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