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pGEX Zap70(C)-SH2

Plasmid

#46533

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Zap70(C) (ZAP70 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 154-267

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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p3F-Strep-hMIB2

Plasmid

#33313

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

MIB2 (MIB2 Human)

Tags

3xFlag and StrepOne

Expression

Mammalian

Promoter

EF1a

Available Since

March 26, 2012

Availability

Academic Institutions and Nonprofits only
pHH0103_MAPK3_Pkinase

Plasmid

#110023

Purpose

Protein expression and purification of MAPK3_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

MAPK3_Pkinase (MAPK3 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_AP2M1_Adap-comp-sub

Plasmid

#109855

Purpose

Protein expression and purification of AP2M1_Adap-comp-sub

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AP2M1_Adap-comp-sub (AP2M1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNM2_Dynamin-N

Plasmid

#110008

Purpose

Protein expression and purification of DNM2_Dynamin-N

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DNM2_Dynamin-N (DNM2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX CblC-SH2

Plasmid

#46414

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Cblc CBLC (CBLC Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 8-326

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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Clover-Zyxin-6

Plasmid

#56531

Purpose

Localization: Focal Adhesions, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Zyxin (ZYX Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pYPQ131-STU-As

Plasmid

#138094

Purpose

Golden Gate entry vector for 1st crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pT2EFCRET(#324)

Plasmid

#184059

Purpose

cyclofen-inducible CRE activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

CRE-ERT2

Use

Zebrafish transient transgenesis

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Xl-EF1a

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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ELF2_pet28a

Plasmid

#131662

Purpose

Bacterial expression of ETS gene ELF2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF2 (ELF2 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMKO shRNA Bmf

Plasmid

#17236

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf shRNA (BMF Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pBC2T-Ypet-FtnA

Plasmid

#108969

Purpose

expresses BC2-tag-Ypet-FtnA in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

FtnA (ftnA Escherichia coli)

Tags

BC2-tag and Ypet

Expression

Mammalian

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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miniSOG-Clathrin-15

Plasmid

#57764

Purpose

Localization: Clathrin Vesicles, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Clathrin (CLTB Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pHH0103_ABLIM1_LIM

Plasmid

#109880

Purpose

Protein expression and purification of ABLIM1_LIM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ABLIM1_LIM (ABLIM1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-H2B-C-10

Plasmid

#55090

Purpose

Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

H2B (H2BC11 Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pGEX Grb7-SH2

Plasmid

#46444

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb7 (GRB7 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 417-532

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Blk-SH2

Plasmid

#46407

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Blk (BLK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 119-224

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX p85b(N)-SH2

Plasmid

#46467

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(N) (PIK3R2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 318-431

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pmCherry-VIM-BC2T

Plasmid

#108966

Purpose

expresses mCherry-vimentin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

VIM (VIM Human)

Tags

BC2T and mCherry

Expression

Mammalian

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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p13xLexAOP2-IVS-Syn21-Voltron-p10

Plasmid

#119042

Purpose

LexAop2-driven expression of Voltron in Drosophila

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Expression

Insect

Promoter

hsp70

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX p85b(C)-SH2

Plasmid

#46466

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(C) (PIK3R2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 610-728

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-10a-Decoy.hPGK.GFP.WPRE

Plasmid

#46581

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-10a Decoy (MIR10A, Mir10a Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pGEX Socs4-SH2

Plasmid

#46507

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs4 (SOCS6 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 376-498

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX tensin-SH2

Plasmid

#46525

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

tensin (TNS1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1451-1588

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5

Plasmid

#117135

Purpose

Expression vector for Nkx2-5 and gRNA

Depositor

Kosuke Yusa

Article

Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

Insert

Nkx2-5 CDS (Nkx2-5 Mouse)

Use

CRISPR and Lentiviral

Mutation

modified BFP

Promoter

human U6 promoter, human EF1a promoter

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Tags

cmyc tag

Expression

Yeast

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Zap70(N)-SH2

Plasmid

#46534

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Zap70(N) (ZAP70 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 1-116

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pJT131_GalL_cCDA1-NGBE3

Plasmid

#145095

Purpose

Expressing base editor cCDA1-NGBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-NGBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO_PSMB2_sgRNA_1

Plasmid

#74184

Purpose

lentiviral vector expressing sgRNA targeting PSMB2

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMB2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_RXRA_Hormone-recep

Plasmid

#109850

Purpose

Protein expression and purification of RXRA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RXRA_Hormone-recep (RXRA Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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