Addgene

We narrowed to 21,567 results for: ESC;

Showing: 7901 - 7920 of 21567 results

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pEB1-Venus SX

Plasmid

#103990

Purpose

Plasmid encoding Venus SX

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Venus SX

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus JBC

Plasmid

#103987

Purpose

Plasmid encoding mVenus JBC

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus JBC

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Lifeact-rsLOV2 pcDNA3.1(+)

Plasmid

#107882

Purpose

Mammalian expression of Lifeact-rsLOV2

Depositor

Stefan Hell

Article

Gregor et al Sci Rep. 2018 Feb 9;8(1):2724. doi: 10.1

Insert

rsLOV2

Use

Tags

Lifeact

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Paxillin-22

Plasmid

#58024

Purpose

Localization: Focal Adhesions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Paxillin

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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p15aC-4D1D

Plasmid

#107864

Purpose

Bacterial expression of PIS and PI4K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-kinase β

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

proD

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJAT13

Plasmid

#18986

Depositor

Jay Keasling

Article

Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

Insert

promoter CP13

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 8, 2008

Availability

Academic Institutions and Nonprofits only
pFA6a-link-yoClover-SpHis5

Plasmid

#44857

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoClover

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pKS043

Plasmid

#65471

Purpose

pTrc plasmid expressing clpS

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

clpS (clpS )

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pJK667

Plasmid

#71690

Purpose

Produces E. coli acetate kinase with an N-terminal His6 tag (H6AckA)

Depositor

T. J. Kappock

Article

Murphy et al Front Chem. 2016 May 23;4:23. doi: 10.33

Insert

acetate kinase (ackA Escherichia coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p)

Plasmid

#70212

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

tetR

Use

Tags

Expression

Mutation

Promoter

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro- TRF2 deltaB deltaM

Plasmid

#18013

Depositor

Titia de Lange

Article

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pKS044

Plasmid

#65472

Purpose

pTrc plasmid expressing clpP

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

clpP (clpP )

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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(GGS)9_(+36GFP)-Cre.ape

Plasmid

#71747

Purpose

+36-GFP-Cre fusion expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_+36GFP-Cre

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAG374-H2B-greenFAST

Plasmid

#160359

Purpose

Expresses H2B-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

H2B-greenFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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plko-puromycin-shTRX1-259

Plasmid

#110921

Purpose

To knockdown Thioredoxin-1

Depositor

Priyamvada Rai

Article

Young et al Biochem Biophys Res Commun. 2010 Feb 12;

Insert

TRX1 (TXN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

unknown

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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SepOTSκ

Plasmid

#68291

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 4xtRNA-Sep-A37

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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AeU6 3p3 GFP LgRNA

Plasmid

#182208

Purpose

For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

3xP3-GFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6

Available since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomWasabi-SpHis5

Plasmid

#44850

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mWasabi with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomWasabi

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro4GFP

Plasmid

#82368

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro4GFP

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/S65T/Q80R/N149C/S202C

Promoter

T7

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pFTSG

Plasmid

#162389

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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