We narrowed to 13,276 results for: cDNA

1. pDEST40-2XHA-Src-S4-KD

   Plasmid

   #140296

   Purpose

   Expresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   contains only the SH4 and KD regions

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST40-2XHA-Src-S4-UD-KD

   Plasmid

   #140300

   Purpose

   Expresses HA-tagged human Src-S4-UD-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   lacks the SH3 and SH2 domains

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDEST47-ARL8A

   Plasmid

   #67466

   Purpose

   Mammalian expression of hArl8 with stop codon (native expression)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8A (ARL8A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pTE3987

   Plasmid

   #137716

   Purpose

   Expresses dLbCas12a-RVRR-BE in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   dLbCas12a-RVRR-BE
   

   Use

   Tags

   6xHis, APOBEC-1, SV40 NLS, and UGI

   Expression

   Mammalian

   Mutation

   G532A, K538V, Y542R, K595R

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST47-ARFRP1

   Plasmid

   #67471

   Purpose

   Mammalian expression of hArfrp with stop codon (native expression)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARFRP1 (ARFRP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. hMEF2A K403R

   Plasmid

   #118355

   Purpose

   This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hMEF2A (MEF2A Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K403R

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST47-ARL5B-GFP

   Plasmid

   #67400

   Purpose

   Mammalian expression of hArl5b with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL5B (ARL5B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pIKAT111

   Plasmid

   #69996

   Purpose

   Mammalian expression of cytochrome p450 CYP2B6 variant

   Depositor

   Kathrin Klein

   Article

   Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

   Insert

   CYP2B6 (CYP2B6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C1459T (R487C)

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRR.6

   Plasmid

   #69997

   Purpose

   Mammalian expression of cytochrome p450 CYP2B6 variant

   Depositor

   Kathrin Klein

   Article

   Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

   Insert

   CYP2B6 (CYP2B6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q172H, K262R

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmyc-GFP-TNRC6A-NLS/NES-mut

    Plasmid

    #42002

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    R930A, R931A, R932A, R934A, F951A, F955A, I958A, …

    Promoter

    CMV

    Available Since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FR_HNF1B_A263insGG

    Plasmid

    #31103

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF1B (HNF1B Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    mutation A263insGG

    Promoter

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FLAG-SENP2 C548A

    Plasmid

    #126592

    Purpose

    Expresses siRNA resistant SENP2 (catalytic dead) in mammalian cells, Dox inducible in TetR cell lines

    Depositor

    Joanna Morris

    Article

    Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

    Insert

    SENP2 (SENP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C548A

    Promoter

    CMV

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C211

    Plasmid

    #174360

    Purpose

    For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (PABPC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …

    Promoter

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST40-ARL1-V5-His6

    Plasmid

    #67437

    Purpose

    Mammalian expression of hArl1 with C-term V5 and His6 tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL1 (ARL1 Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    bp 231 C to T; silent mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. B9L-BirA*

    Plasmid

    #109146

    Purpose

    To generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

    Depositor

    Mariann Bienz

    Article

    van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

    Insert

    B9L (Bcl9l Mouse)
    

    Use

    Tags

    BirA(R118G) and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRD397

    Plasmid

    #163104

    Purpose

    Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mTurquoise2_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FR_HNF1B_P328L329del

    Plasmid

    #31102

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF1B (HNF1B Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    mutation P328L329delCCTCT

    Promoter

    Available Since

    Oct. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CNC3G (CASANOVA-C3 with glycine linkers)

    Plasmid

    #137192

    Purpose

    Expresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory protein

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

    Insert

    CNC3G (CASANOVA-C3 with glycine linkers)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1029-AIDx-CP

    Plasmid

    #135341

    Purpose

    Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1029 nSpyCas9-hAIDx
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Opto-GPR173

    Plasmid

    #106058

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR173; Opto-GPR173; E12)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR173 (GPR173 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDEST47-ARL1

    Plasmid

    #67458

    Purpose

    Mammalian expression of hArl1 with stop codon (native expression)

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL1 (ARL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    bp 231 C to T; silent mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pDEST40-ARL8A-V5-His6

    Plasmid

    #67445

    Purpose

    Mammalian expression of hArl8 with C-term V5 and His6 tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8A (ARL8A Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pDEST40-ARF3-V5-His6

    Plasmid

    #67433

    Purpose

    Mammalian expression of hArf3 with C-term V5 and His6 tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF3 (ARF3 Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

24. Opto-GPR31

    Plasmid

    #106014

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR31; Opto-GPR31; B4)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR31 (GPR31 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. RF-118-UB-BRCA1

    Plasmid

    #78960

    Purpose

    Expression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cells

    Depositor

    Richard Fahlman

    Article

    Xu et al J Biol Chem. 2012 Mar 2;287(10):7495-502

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    3x Flag and ubiquitin

    Expression

    Mammalian

    Mutation

    delta 1-1155

    Promoter

    CMV

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDEST40-2XHA-Src-S4-UD-S32

    Plasmid

    #140302

    Purpose

    Expresses HA-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    lacks the KD region

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDEST40-2XHA-Src-S4-S32-KD

    Plasmid

    #140298

    Purpose

    Expresses HA-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    lacks the UD region

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FLAG-SENP2 WT

    Plasmid

    #126591

    Purpose

    Expresses siRNA resistant SENP2 (WT) in mammalian cells, Dox inducible in TetR cell lines

    Depositor

    Joanna Morris

    Article

    Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

    Insert

    SENP2 (SENP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    siRNA resistant to GAGGAGAUAUUCAGACAUU

    Promoter

    CMV

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGAL4-DBD-TBX1wt-IDR

    Plasmid

    #145254

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and TBX1wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBX1wt-IDR (TBX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDEST40-ARL8B-V5-His6

    Plasmid

    #67446

    Purpose

    Mammalian expression of hArl8b with C-term V5 and His6 tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only