12,913 results

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pISH-Vmn2r76-VR2

Plasmid

#108085

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r76 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r76 (Vmn2r76 Mouse)

Use

In vitro transcription

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Vmn2r118

Plasmid

#108086

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r118 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r118 (Vmn2r118 Mouse)

Use

In vitro transcription

Mutation

changed T to C at nt 830 of NM_001104582.1

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Vmn2r120-TR

Plasmid

#108087

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r120 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r120 (Vmn2r120 Mouse)

Use

In vitro transcription

Mutation

changed A to G at nt 1196 of NM_001104591.1

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-mTRAF3ip3∆TM-CFP

Plasmid

#125182

Purpose

Expresses mouse TRAF3IP3 ∆TM in mammalian cells

Depositor

Shao-Cong Sun

Insert

TRAF3ip3 (Traf3ip3 Mouse)

Tags

CFP

Expression

Mammalian

Mutation

K87R and S359P- See depositor comments below

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-HA-Tbkbp1 1-280

Plasmid

#125178

Purpose

Retroviral expression of mouse Tbkbp1 (1-280)

Depositor

Shao-Cong Sun

Article

Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

Insert

Tbkbp1 (Tbkbp1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Contains amino acids 1-280

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Nkx3-2_Sense

Plasmid

#124437

Purpose

Plasmid for sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

NK3 homeobox 2 (Nkx3-2 Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Gli3_Sense

Plasmid

#124439

Purpose

Plasmid for sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

GLI-Kruppel family member GLI3 (Gli3 Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Rab28_Sense

Plasmid

#124441

Purpose

Plasmid for sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

RAB28, member RAS oncogene family (Rab28 Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Rab28_Anti-Sense

Plasmid

#124442

Purpose

Plasmid for anti-sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

RAB28, member RAS oncogene family (Rab28 Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Bod1l_Sense

Plasmid

#124443

Purpose

Plasmid for sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-HA-Tbkbp1 1-330

Plasmid

#125179

Purpose

Retroviral expression of mouse Tbkbp1 (1-330)

Depositor

Shao-Cong Sun

Article

Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

Insert

Tbkbp1 (Tbkbp1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Contains amino acids 1-330

Promoter

CMV

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3/S3

Plasmid

#124627

Purpose

Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 237 to 241 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-S3

Plasmid

#124550

Purpose

Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 242 to 246 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS-H2

Plasmid

#123358

Purpose

Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 207 to 215 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5

Plasmid

#117137

Purpose

Expression vector encoding BFP-BSD-Nkx2-5

Depositor

Kosuke Yusa

Article

Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

Insert

Nkx2-5 CDS (Nkx2-5 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

modified BFP

Promoter

human U6 promoter, human EF1a promoter

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP

Plasmid

#124181

Purpose

piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

IRES-Pac, Pou5f1 enhancer

Use

Reporter

Promoter

UBCmin

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pZDonor-U6-myogenin stop codon gRNA

Plasmid

#69549

Purpose

Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.

Depositor

Charles Gersbach

Insert

Myogenin C terminus-targeting guide RNA

Use

CRISPR

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMX-eSOX2AHK

Plasmid

#118826

Purpose

Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

Depositor

Ralf Jauch

Article

Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

Insert

eSox2AHK (Sox2 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

AHK: E46A, I53H

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMX-eSOX2NRR

Plasmid

#118825

Purpose

Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

Depositor

Ralf Jauch

Article

Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

Insert

eSOX2NRR (Sox2 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

NRR: E46N, I53R, K57R

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7

Plasmid

#118614

Purpose

expression of mChe-tagged cdc42E7 under doxy-inducible CMV promoter

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

tetON CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1753_S2F-IMCg_doxy-CMV_mChe-Cdc42E7-E63'UTR

Plasmid

#118615

Purpose

expression of mChe-tagged cdc42E7-E6-3'UTR (swap) under doxy-inducible CMV promoter

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7-E6 3'UTR (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

tetON CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1809_pBS_cdc42E6-boxB

Plasmid

#118609

Purpose

cdc42E6 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E6 3'UTR (Cdc42 Mouse)

Use

In vitro transcription

Tags

boxB

Promoter

T3

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1807_pBS_cdc42E7-boxB

Plasmid

#118612

Purpose

cdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 3'UTR (Cdc42 Mouse)

Use

In vitro transcription

Tags

boxB

Promoter

T3

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

Plasmid

#124180

Purpose

piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

EGFP-IRES-Pac, Pou5f1 enhancer

Use

Reporter

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

Plasmid

#119229

Purpose

Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.

Depositor

Kari Alitalo

Insert

IL3SP-Vegfd-dNdC (Vegfd Mouse)

Use

AAV

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSiren_EGFP-shHmga2#2

Plasmid

#122290

Purpose

Knockdown for mouse Hmga2

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for mouse Hmga2 (Hmga2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHTSHP-ShhN-Cys

Plasmid

#121136

Purpose

Expresses ShhN with a C-terminal cysteine residues in bacteria

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

shh (Shh Mouse)

Tags

HIS, HRV3C site, and MBP

Expression

Bacterial

Mutation

Amino acid sequence from 26-191, with an addition…

Promoter

tac

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 FLAG membrane-bound lipin-1 (delta200)

Plasmid

#120278

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the delta200 Golgi-…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-REEP1/2 [N326D/29R]

Plasmid

#114510

Purpose

Mammalian Expression Plasmid of anti-REEP1/2 (Mouse). Derived from hybridoma N326D/29.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-REEP1/2 (Mus musculus) recombinant mouse monoclonal antibody (Reep1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-KCNT2/Slo2.1/Slick K+ channel [N11/33R]

Plasmid

#114496

Purpose

Mammalian Expression Plasmid of anti-KCNT2/Slo2.1/Slick K+ channel (Mouse). Derived from hybridoma N11/33.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-KCNT2/Slo2.1/Slick K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnt2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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