Showing: 61 - 80 of 3546 results
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Plasmid#135233Purposeconstitutive expression of HY5 from A. thaliana (AT5G11260; downregulates UPR signaling)DepositorInsertHY5
UseTagsExpressionPlantMutationPromoter35SAvailabilityAcademic Institutions and Nonprofits only -
p35S::mCh-HA-P2A-lifeact-mCh
Plasmid#135234Purposeconstitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)DepositorInsertmCh-HA-P2A-lifeact-mCh
UseTagsHA, lifeact, and p2aExpressionPlantMutationPromoter35SAvailabilityAcademic Institutions and Nonprofits only -
pRGEB33
Plasmid#158151PurposeConstruction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRGEB34
Plasmid#158152PurposeConstruction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p32Fn
Plasmid#158153PurposeConstruct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationRice codon optimizedPromoterAvailabilityAcademic Institutions and Nonprofits only -
p32Lb
Plasmid#158154PurposeConstruct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p33Fn
Plasmid#158155PurposeExpression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationRice codon optimizedPromoterAvailabilityAcademic Institutions and Nonprofits only -
p33Lb
Plasmid#158156PurposeExpression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRWJB004
Plasmid#160112PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10AvailabilityAcademic Institutions and Nonprofits only -
pRWJB006
Plasmid#160113PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailabilityAcademic Institutions and Nonprofits only -
pICH47802-p35S::tdTomato-ER::tNOS
Plasmid#160220PurposetdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverseDepositorInsertp2x35S::TdTomato-ER::tNOS
UseSynthetic Biology; Moclo toolkit level 1 acceptor…TagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pICH47811-pAtAct2::Cas9-nls::tSlATPase
Plasmid#160221PurposeCas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverseDepositorInsertpAtACT2::Cas9-nls::tSlATPase
UseSynthetic Biology; Moclo toolkit level 1 acceptor…TagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-RVRR
Plasmid#160327PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V, N573R and K625R mutations, without promoterDepositorInsertMb2Cas12a RVRR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationN563R, K569V, N573R and K625RPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUPD2 hpIntron (GB1281)
Plasmid#160569PurposeIntron sequence from S. lycopersicumDepositorInserthpIntron
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 35s:PIF6:tNos (GB0170)
Plasmid#160606PurposeTU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switchDepositorInsertPIF6
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailabilityAcademic Institutions and Nonprofits only -
pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)
Plasmid#160607PurposeTU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.DepositorInsertCUP2Gal4AD
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)
Plasmid#160608PurposeTU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)DepositorInsertP35s:dCas9:SRDX:tNOS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)
Plasmid#160609PurposeTU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64DepositorInsertP35S:MS2-VP64:Tnos
UseTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha2 P35S:RDF:T35S (GB1508)
Plasmid#160613PurposeTU for the constitutive expression of Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)DepositorInsertRDF
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)
Plasmid#160614PurposeTU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.DepositorInsertPhiC31
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromotermini35sAvailabilityAcademic Institutions and Nonprofits only
Showing: 61 - 80 of 3546 results