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pRRL-Vinculin_TurboID_Venus

Plasmid

#200212

Purpose

Lentivirus vector expressing vinculin with co-localization labeler TurboID and fluorescence reporter Venus

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

Vinculin_TurboID_Venus

Use

Lentiviral

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

Plasmid

#218866

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8f

Use

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP5G

Plasmid

#31788

Purpose

a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

Depositor

Douglas Kim, Loren Looger, GENIE Project

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

GCaMP5G

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-TVA-IRES-Puro

Plasmid

#172366

Purpose

Generation of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + Puro

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8m

Plasmid

#169248

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

Plasmid

#105323

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7c variant 1513

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only
pRRL-VinculinTS

Plasmid

#111830

Purpose

Vinculin tension sensor in lentiviral expression vector

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (VCL Chicken)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jGCaMP8m

Plasmid

#172719

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAc-Neo-OVA

Plasmid

#22533

Depositor

Michael Bevan

Article

Moore et al Cell. 1988 Sep 9. 54(6):777-85.

Insert

OVA (OVAL Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-OTp-GCaMP6s

Plasmid

#192945

Purpose

To drive GCaMP6s under the control of mouse oxytocin promoter

Depositor

Kazunari Miyamichi

Article

Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Ii-OVA.I+II

Plasmid

#64596

Purpose

Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation

Depositor

Sandra Diebold, Martin Zenke

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Use

Tags

Invariant chain

Expression

Mammalian

Mutation

Only amino acids 242-353 (class I and class II ep…

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGEM cCol2 (CT#617)

Plasmid

#13943

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

Collagen II (COL2A1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Nucleotides 182-616

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
NINJA TC

Plasmid

#159274

Purpose

Expresses the NINJA targeting construct (inducible neoantigen expression via cumulative Cre, rtTA, doxycycline and tamoxifen).

Depositor

Nikhil Joshi

Article

Damo et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

NINJA TC

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM cAgg (CT#689)

Plasmid

#13945

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

Aggrecan (ACAN Chicken)

Use

Tags

Expression

Bacterial

Mutation

Nucleotides 113-800

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pSK+ chickBMPR-1B DN

Plasmid

#49529

Purpose

cloning vector for dominant negative chick BMP receptor 1B

Depositor

Lee Niswander, Peter ten Dijke

Article

Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

Insert

BMPR-1B DN (BMPR1B Chicken)

Use

Tags

Expression

Bacterial

Mutation

dominant negative mutation (K231R); also P2A, M11…

Promoter

T3

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pET-21a(+)-AK1-His

Plasmid

#118977

Purpose

Bacterial expression plasmid for Adenylate kinase isoenzyme 1

Depositor

Sebastian Maerkl

Article

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcSrc527

Plasmid

#17675

Depositor

David Shalloway

Article

Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Insert

c-src Y527F (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Tyr 527 changed to Phe . Activates transforming a…

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

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mCherry-C-Src-7

Plasmid

#55002

Purpose

Localization: Membrane/Vesicles, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

C-Src

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
Paxillin-pEGFP

Plasmid

#15233

Depositor

Rick Horwitz

Article

Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

Insert

paxillin (PXN Chicken)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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mCherry-C-Src-DN-7

Plasmid

#55003

Purpose

Localization: Membrane/Vesicles, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

C-Src DN

Use

Tags

mCherry

Expression

Mammalian

Mutation

Kinase Dead K295R

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only