We narrowed to 6,273 results for: nls
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Plasmid#239024PurposeCo-overexpression of Rhobin2-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling markerDepositorInsertRhobin2-SEC61B (SEC61B Synthetic, Human)
TagsTagBFPExpressionMammalianPromoterCMV promoterAvailable SinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFAP-NLS-CasRx-NLS-FLAG
Plasmid#154000PurposeExpress CasRx for AAV packageDepositorInsertCasRx
UseAAV and CRISPRTagsFLAGPromoterGFAPAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dMisCas13b-SNAPf-NLS-3xFlag
Plasmid#191536PurposeOverexpression of NLS-dMisCas13b-SNAPf-NLS-3xFlag in human cellsDepositorInsertNLS-dMisCas13b-SNAPf-NLS-3xFlag
ExpressionMammalianAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His
Plasmid#171587PurposeBacterial expression of NLS-RfxCas13d-NLS 6HisDepositorInsertMKIEE-NLS-RfxCas13d-NLS-HA-6His
TagsHA-6HIS and MKIEEExpressionBacterialAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
nCre-ST-NLS-P2A-NLS-pSC-cCre
Plasmid#207638PurposeA plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequenceDepositorInsertnCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
ExpressionMammalianMutationAmber stop codon at SpyCatcherās critical lysinePromoterCMVAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSt1374-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS
Plasmid#112210PurposeTargeted DNA methylationDepositorAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSt1374-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS
Plasmid#112209PurposeTargeted DNA methylationDepositorAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLS-nCas9-NLS-P2A-EGFP (pRZ70)
Plasmid#123616PurposeCMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).DepositorInsertNLS-nCas9-NLS-P2A-EGFP
ExpressionMammalianMutationD10A in SpCas9PromoterCMVAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS
Plasmid#121835PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1
Plasmid#121824PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9/LSD1 (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268
Plasmid#69219PurposeExpresses wild type SpCas9 fused to Zif268 in mammalian cellsDepositorAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4
Plasmid#69227PurposeExpresses wild type SpCas9 fused to ZFP-TS4 in mammalian cellsDepositorInsertTS4 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2
Plasmid#69225PurposeExpresses wild type SpCas9 fused to ZFP-TS2 in mammalian cellsDepositorInsertTS2 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS
Plasmid#121834PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-Cas9(3.7) (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3
Plasmid#69226PurposeExpresses wild type SpCas9 fused to ZFP-TS3 in mammalian cellsDepositorInsertTS3 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS
Plasmid#121828PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7) (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS
Plasmid#69223PurposeExpresses R1335K mutant SpCas9 in mammalian cellDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianMutationArginine 1335 is changed to LysineAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAG-NLS-mScarleti-NLS-IRES-Puromycin
Plasmid#234890PurposeExpressed a nuclear mScarleti fluorophoro in mammalian cellsDepositorInsertmScarleti
TagsNLS on N- and C- terminalsExpressionMammalianPromoterCAGAvailable SinceSept. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTOMM20-Rhobin2-IRES-NLS-mTagBFP-2xNLS
Plasmid#239022PurposeCo-overexpression of TOMM20-Rhobin2 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling markerDepositorInsertTOMM20-Rhobin2 (TOMM20 Synthetic, Human)
TagsTagBFPExpressionMammalianPromoterCMV promoterAvailable SinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTOMM20-Rhobin9-IRES-NLS-mTagBFP-2xNLS
Plasmid#239023PurposeCo-overexpression of TOMM20-Rhobin9 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling markerDepositorInsertTOMM20-Rhobin9 (TOMM20 Synthetic, Human)
TagsTagBFPExpressionMammalianPromoterCMV promoterAvailable SinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRhobin9-SEC61B-IRES-NLS-mTagBFP-2xNLS
Plasmid#239025PurposeCo-overexpression of Rhobin9-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling markerDepositorInsertRhobin9-SEC61B (SEC61B Synthetic, Human)
TagsTagBFPExpressionMammalianPromoterCMV promoterAvailable SinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS
Plasmid#169596PurposeTier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).DepositorInsertdead S.pyogenes Cas9
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core
Plasmid#121825PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9/p300core (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L
Plasmid#121826PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9/Dnmt3a-3L (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPR
Plasmid#121822PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9/VPR (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IB801: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-KRAB
Plasmid#121823PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9/KRAB (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT1-NLS-3XHA-NLS
Plasmid#69221PurposeExpresses R1333K mutant SpCas9 in mammalian cellDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianMutationArginine 1333 is changed to LysineAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT2-NLS-3XHA-NLS
Plasmid#69222PurposeExpresses R1333S mutant SpCas9 in mammalian cellDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianMutationArginine 1333 is changed to SerineAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)
Plasmid#114082PurposeCAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348DepositorInsertDNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
Tags2x SV40 NLS and SV40 NLSExpressionMammalianMutationE174R, S542R, K548R and D908A, human codon optimiā¦Available SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH
Plasmid#178960PurposeExpresses saCas9 protein specifically in astrocytesDepositorInsertStaphylococcus aureus Cas9 (SaCas9)
UseAAVTags3x HAPromotergfaABC1D (astrocyte specific)Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only