We narrowed to 16,752 results for: EMB

1. pRK5 FLAG membrane-bound lipin-1 (delta200)

   Plasmid

   #120278

   Purpose

   Expression of ER/Golgi-tethered lipin-1 in mammalian cells

   Depositor

   Sirio Dupont

   Article

   Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

   Insert

   Lipin-1 (Lpin1 Mouse)
   

   Use

   Tags

   FLAG and in-frame fusion with the delta200 Golgi-…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Plasmid

   #163043

   Purpose

   QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   QuasAr2
   

   Use

   Lentiviral

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

   Plasmid

   #163063

   Purpose

   Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   CheRiff
   

   Use

   Gateway entry vector

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

   Plasmid

   #158585

   Purpose

   Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

   Depositor

   Cynthia Kenyon

   Article

   Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

   Insert

   membrane localization signal-tagBFP-wrmScarlet11
   

   Use

   Tags

   tagBFP

   Expression

   Yeast

   Mutation

   GGGS linker

   Promoter

   TEF

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Plasmid

   #163062

   Purpose

   Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   QuasAr2
   

   Use

   Gateway entry vector

   Tags

   mOrange2

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX Supt6h-SH2

   Plasmid

   #46518

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Supt6h (SUPT6H Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1317-1441

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag-hCRM1

   Plasmid

   #17647

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   CRM1 (XPO1 Human)
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-Expotin 1

   Plasmid

   #42047

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   Exportin-1 (XPO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET29b Tau Cys

   Plasmid

   #108867

   Purpose

   Expresses human Tau with a mutated cysteine

   Depositor

   Gabriele Kaminski Schierle

   Insert

   Tau (MAPT Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I260C C291A C322A to allow maleimide dye labelling

   Promoter

   T7

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. His8-TEV-PseC

    Plasmid

    #89724

    Purpose

    Expresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage site

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    PseC (pseC Campylobacter jejuni)
    

    Use

    Tags

    His8 and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

11. His8-TEV-PseH

    Plasmid

    #89725

    Purpose

    Expresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage site

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    PseH (pseH Campylobacter jejuni)
    

    Use

    Tags

    2xHis Gln 5xHis and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pISH-Pou6f1

    Plasmid

    #74356

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pou6f1. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    POU domain, class 6, transcription factor 1 (Pou6f1 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEMT-mMyh3CDS

    Plasmid

    #192843

    Purpose

    Plasmid to produce ISH probe for mMyh3 gene with CDS sequence

    Depositor

    Masafumi Inui

    Article

    Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

    Insert

    myosin heavy chain 3 (Myh3 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEMT-mMyh3UTR

    Plasmid

    #192844

    Purpose

    Plasmid to produce ISH probe for mMyh3 gene with UTR sequence

    Depositor

    Masafumi Inui

    Article

    Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

    Insert

    myosin heavy chain 3 (Myh3 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAPM-miR30-XPO1-ts1

    Plasmid

    #174286

    Purpose

    XPO1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    XPO1 shRNA (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAPM-miR30-XPO1-ts2

    Plasmid

    #174287

    Purpose

    XPO1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    XPO1 shRNA (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAPM-miR30-XPO1-ts3

    Plasmid

    #174288

    Purpose

    XPO1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    XPO1 shRNA (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SSB-wt

    Plasmid

    #197260

    Purpose

    wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

    Depositor

    Timothy Lohman

    Article

    Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

    Insert

    wt E. coli SSB
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a MISTIC

    Plasmid

    #85999

    Purpose

    bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    N/A
    

    Use

    Tags

    HIS6 and MISTIC

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mEos3.2trLATm2allL+A

    Plasmid

    #203750

    Purpose

    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLATm2(allL+A) (LAT Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. C1-CFP-Lyn11

    Plasmid

    #155234

    Purpose

    membane marker

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lyn11 membrane targeted CFP. Membrane control
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PCMV-intron myc Rab1aWT

    Plasmid

    #46776

    Purpose

    expression of WT Rab1a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    Rab1a (RAB1A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pQCXIP GFP-MOSPD2 WT

    Plasmid

    #186467

    Purpose

    Expression of human MOSPD2 fused to EGFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PCMV-intron myc Rab6WT

    Plasmid

    #46781

    Purpose

    expression of WT Rab6a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    Rab6 (RAB6A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3 Flag Rab1a N124I

    Plasmid

    #46778

    Purpose

    expression of N124I Rab1a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    Rab1a (Rab1a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N124I cannot stably bind guanine nucleotide

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pSI HA Nup53xl

    Plasmid

    #86000

    Purpose

    mammalian expression of HA tagged Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    Xenopus laevis Nup53 (nup35.L Frog)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimzed for bacterial expression

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSI myc Nup53xl

    Plasmid

    #86001

    Purpose

    mammalian expression of myc tagged Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    Xenopus laevis Nup53 (nup35.L Frog)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    codon optimzed for bacterial expression

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a SUMO Nup53xl 1-320

    Plasmid

    #85998

    Purpose

    bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    Xenopus laevis Nup53 (nup35.L Frog)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PCMV-intron myc Rab1a S25N

    Plasmid

    #46777

    Purpose

    expression of S25N Rab1a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    Rab1a S25N mutant (RAB1A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S25N (dominant negative)

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

30. PCMV-intron myc Rab6 T27N

    Plasmid

    #46782

    Purpose

    expression of T27N Rab6a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    Rab6 T27N (RAB6A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    T27N dominant negative

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

31. Rab8WT(Flag) in pcDNA3.1neo

    Plasmid

    #46783

    Purpose

    expression of WT Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A (RAB8A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

32. pQCXIP GFP-MOSPD2 RD/LD

    Plasmid

    #186468

    Purpose

    Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to EGFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R404D/L406D (mutant unable to bind FFAT motifs)

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PCMV-intron myc Rab11WT

    Plasmid

    #46785

    Purpose

    expression of WT Rab11a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

34. trLATm1PtoLmEos3.2

    Plasmid

    #203749

    Purpose

    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Prolines P8 and P17 in the LAT transmembrane domain mutated to L. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLATm1 (PtoL) (LAT Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    P8L, P17L

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET15b MOSPD2 [282-490]

    Plasmid

    #186469

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [282-490]

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pmCherryC1 VAP-A

    Plasmid

    #226407

    Purpose

    Expression of human VAP-A fused to mCherry in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    VAP-A (VAPA Human)
    

    Use

    Tags

    T7-Xpress tag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3 TFAM-mClover

    Plasmid

    #129574

    Purpose

    Expression of mClover fused TFAM

    Depositor

    Stephen Tait

    Article

    Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3 TFAM-mScarlet

    Plasmid

    #129573

    Purpose

    Expression of mScarlet fused TFAM

    Depositor

    Stephen Tait

    Article

    Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmCherryC1 VAP-B

    Plasmid

    #226409

    Purpose

    Expression of human VAP-B fused to mCherry in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    VAP-B (VAPB Human)
    

    Use

    Tags

    HA, T7-Xpress tag, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PCMV-intron myc Rab11 S25N

    Plasmid

    #46786

    Purpose

    expression of S25N Rab11a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB11A S25N (RAB11A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S25N dominant negative

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only