We narrowed to 378 results for: pET-15b

1. pET15b+PCNA3 T180C

   Plasmid

   #66181

   Purpose

   Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 3
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   T180C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET15b+PCNA3 R112C

   Plasmid

   #66180

   Purpose

   Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 3
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET15b+PCNA2 E105C

   Plasmid

   #66176

   Purpose

   Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 2
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E105C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET15b-eIF4E-S200C

   Plasmid

   #37229

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Insert

   eIF4E
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S200C

   Promoter

   T7

   Available since

   Aug. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pET-15b PAI-RBP

   Plasmid

   #45390

   Depositor

   Thomas Gelehrter

   Article

   Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

   Insert

   PAI-RBP (SERBP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pET-15b-TEV-C9R

   Plasmid

   #118754

   Purpose

   TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

   Depositor

   Yutaka Kuroda

   Article

   Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

   Insert

   Tobacco Etch Virus protease (NEWENTRY Synthetic)
   

   Use

   Tags

   C9R and His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-15b-UbcH5b/2419

   Plasmid

   #11475

   Depositor

   Allan Weissman

   Article

   Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

   Insert

   UbcH5b (UBE2D2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   n/a

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pET15b/Shigella flexneri IpaA

   Plasmid

   #46178

   Depositor

   Susan Craig

   Article

   Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

   Insert

   IpaA
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET15b+His SUMO PPM1H

   Plasmid

   #207333

   Purpose

   Bacterial expression of His SUMO PPM1H

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   PPM1H (PPM1H Human)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15b-ACPs (from S. pneumonia)

    Plasmid

    #63687

    Purpose

    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae

    Depositor

    Michael Johnson

    Insert

    acpS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pET15b T7pCONS TIR-2 sfGFP

    Plasmid

    #154468

    Purpose

    pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET15b SARS-CoV PLpro

    Plasmid

    #61693

    Purpose

    inducible expression of SARS PLpro protease

    Depositor

    Michael Johnson

    Article

    Lee et al ChemMedChem. 2013 Aug;8(8):1361-72. doi:

    Insert

    SARS-CoV PLpro
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. Mucin MUC1-5TR-pET15b

    Plasmid

    #174694

    Purpose

    Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

    Depositor

    Jean Gariepy

    Article

    Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

    Insert

    MUC1-TR
    

    Use

    Tags

    hexahistidine (HIS) tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET15b-Casp8 delta DED

    Plasmid

    #11827

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 8 minus Death Effecter Domain (CASP8 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Minus Death Defecter Domain (aa 1-216)

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pET 15b-UbcH5a/2417

    Plasmid

    #11472

    Depositor

    Allan Weissman

    Article

    Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

    Insert

    UbcH5a (UBE2D1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

16. 3521 pET15b Bid p15

    Plasmid

    #8782

    Depositor

    Stanley Korsmeyer

    Article

    Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

    Insert

    Bid p15 (Bid Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pET-tTALE-mStrawberry-15b

    Plasmid

    #206868

    Purpose

    Express tTALE-mStrawberry

    Depositor

    Kyubong Jo

    Article

    Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

    Insert

    7-mer tTALE-mStrawberry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Pet15b-Endosomal Escape Control

    Plasmid

    #186552

    Purpose

    Negative Control confirming the need for a CPP peptide in mediating internalization

    Depositor

    Jean Gariepy

    Article

    Mohammed et al J Control Release. 2012 Nov 28;164(1):58

    Insert

    6H-ETA-GFP
    

    Use

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Pet15b-Internalization and Endosomal Escape

    Plasmid

    #186551

    Purpose

    Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

    Depositor

    Jean Gariepy

    Article

    Mohammed et al J Control Release. 2012 Nov 28;164(1):58

    Insert

    10R-8His-ETA-EGFP
    

    Use

    Tags

    8xHis and EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-tTALE-EmGFP-15b

    Plasmid

    #138280

    Purpose

    Express tTALE-EmGFP

    Depositor

    Kyubong Jo

    Article

    Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

    Insert

    7-mer tTALE-EmGFP
    

    Use

    TALEN

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 3535 pET15b Bid p22

    Plasmid

    #8784

    Depositor

    Stanley Korsmeyer

    Article

    Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

    Insert

    Bid p22 (Bid Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

22. pET 15b-UbcH5c/2418

    Plasmid

    #11473

    Depositor

    Allan Weissman

    Article

    Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

    Insert

    UbcH5c (UBE2D3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    March 8, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pET-TALE-mCherry-15b

    Plasmid

    #138287

    Purpose

    Express tTALE-mCherry

    Depositor

    Kyubong Jo

    Article

    Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

    Insert

    7-mer tTALE-mCherry
    

    Use

    TALEN

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET15b/EYFP-GgVcl 1-851

    Plasmid

    #46264

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

    Insert

    Vcl 1-851 (VCL Chicken)
    

    Use

    Tags

    EYFP and His

    Expression

    Bacterial

    Mutation

    aa 1-851 (aka Vh) head domain

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET15b-Fab1 (Francisella tularensis)

    Plasmid

    #61690

    Purpose

    Inducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensis

    Depositor

    Michael Johnson

    Article

    Hevener et al J Med Chem. 2012 Jan 12;55(1):268-79. do

    Insert

    enoyl-[acyl-carrier-protein] reductase (NADH)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pET15b/GgVcl V1-851 (aka Vh)

    Plasmid

    #46172

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

    Insert

    Vcl 1-851 (VCL Chicken)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-851 (aka Vh) head domain

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET15b-ACP (from S. aureus)

    Plasmid

    #63686

    Purpose

    Inducible expression of Acyl Carrier Protein (ACP) from S. aureus

    Depositor

    Michael Johnson

    Insert

    ACP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

28. M26I DJ-1 TEV site pET15b

    Plasmid

    #60685

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26I DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26I and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pET15b+PCNA3 R112C/T180C

    Plasmid

    #66182

    Purpose

    Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C, T180C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET15b+PCNA2 E105C/L171C

    Plasmid

    #66178

    Purpose

    Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C, L171C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. M26L DJ-1 TEV site pET15b

    Plasmid

    #60686

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26L DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26L and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pET15b/EYFP-GgVcl 1-258

    Plasmid

    #46263

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

    Insert

    Vcl 1-258 (VCL Chicken)
    

    Use

    Tags

    EYFP and His

    Expression

    Bacterial

    Mutation

    aa 1-258 (VD1)

    Promoter

    T7

    Available since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-mNeonGreen-HMG-15b

    Plasmid

    #206872

    Purpose

    Express mNeonGreen-HMG

    Depositor

    Kyubong Jo

    Article

    Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

    Insert

    mNeonGreen-HMG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-tTALE-mTurquoise2-15b

    Plasmid

    #206871

    Purpose

    Express tTALE-mTurquoise2

    Depositor

    Kyubong Jo

    Article

    Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

    Insert

    7-mer tTALE-mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET-tTALE-mNeonGreen-15b

    Plasmid

    #206870

    Purpose

    Express tTALE-mNeonGreen

    Depositor

    Kyubong Jo

    Article

    Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

    Insert

    7-mer tTALE-mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-tTALE-mKO2-15b

    Plasmid

    #206869

    Purpose

    Express tTALE-mKO2

    Depositor

    Kyubong Jo

    Article

    Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

    Insert

    7-mer tTALE-mKO2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET15b-His6-CUbo-GFP

    Plasmid

    #212720

    Purpose

    Bacterial expression of His6-CUbo-GFP

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    GFP
    

    Use

    Tags

    6xHis-CUbo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET15b I-SceI (Ctermhis) K223I

    Plasmid

    #195615

    Purpose

    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity

    Depositor

    Frederick Gimble

    Article

    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

    Insert

    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C2694G, G2345C, G2351C, A3365T, A3366T

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET15b I-SceI (Ctermhis) K122I

    Plasmid

    #195614

    Purpose

    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity

    Depositor

    Frederick Gimble

    Article

    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

    Insert

    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C2458G, A2826T, A2827T

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET-15b-LanCL1-H219A

    Plasmid

    #154190

    Purpose

    To express LanCL1-H219A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL1
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    His219->Ala219

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge