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Showing: 151 - 200 of 1216 results

  1. pScarlessHD-2xHA-DsRed

    Plasmid
    #80822
    Purpose
    Vector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    2xHA-piggyBac-DsRed
    Use
    Unspecified
    Tags
    2xHA
    Expression
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80822-plasmid-map-sequence-id-152787

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  4. PB Neo* PB

    Plasmid
    #138413
    Purpose
    Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Piggybac
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138413-plasmid-map-sequence-id-316185

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  5. pScarlessHD-DsRed-w+

    Plasmid
    #80801
    Purpose
    Vector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    piggyBac-3xP3-DsRed
    GMR-white
    Use
    High copy, amp resistance
    Tags
    Expression
    Mutation
    Promoter
    3xP3 and GMR::hsp70
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80801-plasmid-map-sequence-id-152801

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  9. pB PUb-integrase

    Plasmid
    #183966
    Purpose
    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker
    Depositor
    Eric Marois
    Article
    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:
    Insert
    PhiC31 integrase
    Use
    Piggybac insect transgenesis destination vector
    Tags
    Expression
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183966-plasmid-map-sequence-id-361316

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  15. pB-rtTA

    Plasmid
    #126034
    Purpose
    PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.
    Depositor
    Mauro Calabrese
    Article
    Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do
    Insert
    rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hUbiC
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    126034-plasmid-map-sequence-id-245792

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  32. PB-TO-NFIA.1-SOX9

    Plasmid
    #182310
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Inserts
    NFIA-T2A-SOX9 (NFIA, SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Piggybac
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182310-plasmid-map-sequence-id-359522

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  40. PB-TO-NFIA.1-SOX9 (bi-directional promoter)

    Plasmid
    #182306
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Inserts
    NFIA (NFIA Human)
    SOX9 (SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Piggybac
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-Bi-directional
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182306-plasmid-map-sequence-id-359516

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  49. pPB_EF1a_Gag-EIF4E-TM

    Plasmid
    #212658
    Purpose
    piggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    Use
    Piggybac
    Tags
    EIF4E (W56L, W102L, W166L)
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212658-plasmid-map-sequence-id-420239

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  50. pPB_EF1a_Gag∆MA

    Plasmid
    #212654
    Purpose
    piggyBac vector to express MLV Gag with truncated MA domain
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of MA domain (amino acids 2-215)
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212654-plasmid-map-sequence-id-420275

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Showing: 151 - 200 of 1216 results