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Showing: 61 - 80 of 85 results

  3. SENP2M497A

    Plasmid
    #61088
    Purpose
    cleave the SUMO off when use PETSUMO vector for express fusion protein
    Depositor
    Cynthia Wolberger
    Article
    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033
    Insert
    SENP2M497A catalytic domain (SENP2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M497A; Catalytic domain is residues 364-589
    Promoter
    T7
    Available since
    Jan. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pEW107

    Plasmid
    #232796
    Purpose
    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2ab
    Depositor
    Cynthia Wolberger
    Article
    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1
    Insert
    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) (SET1, SPP1, SWD3 Budding Yeast)
    Use
    Tags
    His6-3xFLAG, Twinstrep
    Expression
    Insect
    Mutation
    ySet1(762-1080)
    Promoter
    polyhedrin promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    232796-plasmid-map-sequence-id-465733

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  16. pTXB1-H2B(1-114)_wt

    Plasmid
    #118252
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Use
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118252-plasmid-map-sequence-id-226720

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  19. pT7-agrBD-IV

    Plasmid
    #53440
    Purpose
    Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV
    Depositor
    Cynthia Collins
    Article
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus (with D29Y mutation in agrD)
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    D to Y mutation of residue 29 of the agrD gene (c…
    Promoter
    T7-lac
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53440-plasmid-map-sequence-id-83235

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Showing: 61 - 80 of 85 results