We narrowed to 11,637 results for: NSI

1. Actin Binding Domain Tension Sensor

   Plasmid

   #215368

   Purpose

   Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   Actin Binding Domain Tension Sensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Actin Binding Domain Tensionless Control

   Plasmid

   #215371

   Purpose

   Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   Actin Binding Domain Tensionless Control
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CIRTS-10: bdefensin 3-SLBP-YTHDF2(1-200)

   Plasmid

   #132546

   Purpose

   Expresses CIRTS-10 (bdefensin 3-SLBP-YTHDF2(1-200)) in mammalian cells

   Depositor

   Bryan Dickinson

   Article

   Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

   Insert

   CIRTS-10: bdefensin 3-SLBP-YTHDF2(1-200)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   cmv d0

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. stabilized HP35 tension sensor module

   Plasmid

   #101251

   Purpose

   Expresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.

   Depositor

   Carsten Grashoff

   Article

   Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

   Insert

   YPet(short)-HP35st-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Desmoplakin II tension sensor (F40-based)

   Plasmid

   #118715

   Purpose

   The F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II-[YPet(short)-F40-mCherry] (internal-1353) (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-F40-mCherry

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   CMV

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2

   Plasmid

   #186361

   Purpose

   Entry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Human Ensconsin (MAP7 Synthetic, Human)
   

   Use

   Expression of a fluorescent microtubule marker

   Tags

   3xEGFP

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Desmoplakin I tension sensor (F40-based)

   Plasmid

   #118725

   Purpose

   The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[YPet(short)-F40-mCherry] (internal-1952) (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-F40-mCherry

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   CMV

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PX408 Francisella tularensis subsp. novicida Cas9

   Plasmid

   #68705

   Purpose

   Francisella tularensis subsp. novicida Cas9

   Depositor

   Feng Zhang

   Insert

   FtCas9
   

   Use

   CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)

   Plasmid

   #49060

   Purpose

   Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Tags

   3x Flag and YFP (chloride-sensitive)

   Expression

   Mammalian

   Mutation

   Cl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…

   Promoter

   CMV

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ldlr (low density lipoprotein receptor)_R (OZ540)

    Plasmid

    #27217

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting ldlr (LOC100329978 Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

11. ldlr (low density lipoprotein receptor)_L (OZ539)

    Plasmid

    #27216

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting ldlr (LOC100329978 Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pSB700 lenti gRNA EBFP2_TtoC and hairpin extension

    Plasmid

    #167921

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSB700 lenti gRNA Blasto_TtoC and hairpin extension

    Plasmid

    #167920

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSB700 lenti gRNA EYFP_TtoC and hairpin extension

    Plasmid

    #167922

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSB700 lenti gRNA Hygro_TtoC and hairpin extension

    Plasmid

    #167923

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSB700 lenti gRNA iRFP713_TtoC and hairpin extension

    Plasmid

    #167924

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSB700 lenti gRNA Puro_TtoC and hairpin extension

    Plasmid

    #167927

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cre-responsive Cas9 expressing transposon library vector

    Plasmid

    #164138

    Purpose

    Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6, EF-1a

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q

    Plasmid

    #132545

    Purpose

    Expresses CIRTS-8 (bdefensin 3-TBP6.7-hADAR(299-701)E488Q) in mammalian cells

    Depositor

    Bryan Dickinson

    Article

    Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

    Insert

    CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q-NLS
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    cmv d0

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

    Plasmid

    #176542

    Purpose

    Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Inserts

    MBP tag
    TnpB
    

    Tags

    3' extension derived from endogenous locus a…

    Expression

    Bacterial

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge