We narrowed to 6,752 results for: poly

1. pDEST-CMV-polysite

   Plasmid

   #122843

   Purpose

   Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Gateway destination

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-LacI-polylinker

   Plasmid

   #179271

   Purpose

   Cloning of GFP-LacI fusion proteins for LacI/LacO tethering

   Depositor

   Lienhard Schmitz

   Article

   Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

   Type

   Empty backbone

   Tags

   GFP and LacI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3E ubb polyA

   Plasmid

   #195977

   Purpose

   Gateway p3E vector

   Depositor

   Alexa Burger, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   ubb:polyA (ubb Zebrafish)
   

   Use

   Other

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-polyQ74-NLS

   Plasmid

   #231892

   Purpose

   Forms nuclear HTT partial exon 1 Q74-NLS aggregates

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q74-NLS (HTT Human)
   

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Mutation

   CAG repeat expansion

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3basic luciferase and renilla_polyA

   Plasmid

   #128046

   Purpose

   pGL3 basic luciferase reporter with co-expression of renilla luciferase

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. T7-CMVtrans-FFLuc-polyA

   Plasmid

   #101156

   Purpose

   Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

   Depositor

   Marcel Bruchez

   Article

   Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Promoter

   T7 promoter

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-polyQ23-NLS

   Plasmid

   #231891

   Purpose

   Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q23-NLS (HTT Human)
   

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.GFAP.SV40.eGFP.SV40(polyA)

   Plasmid

   #195558

   Purpose

   Expresses eGFP under control of short GFAP promoter

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   No tags

   Expression

   Mammalian

   Promoter

   short GFAP

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3e UbB polyA

   Plasmid

   #188702

   Purpose

   Gateway 3' element encoding the ubiquitin B polyadenylation sequence

   Depositor

   David Tobin

   Article

   Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

   Insert

   UbB polyA (ubb Zebrafish)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. HO-pGAL-poly-KanMX4-HO

    Plasmid

    #51664

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Expression

    Yeast

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-HIRA-polyA

    Plasmid

    #59781

    Purpose

    Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    HIRA
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOT2-poly-cis

    Plasmid

    #141177

    Purpose

    To clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoter

    Depositor

    Guiliang Tang

    Type

    Empty backbone

    Use

    RNAi and Synthetic Biology

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. HO-pMET-poly-KanMX4-HO

    Plasmid

    #51665

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoter

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Expression

    Yeast

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. attB-mCh-Poly(A)

    Plasmid

    #65525

    Purpose

    Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-mCherry
    

    Use

    Mouse Targeting; Bxb1

    Expression

    Mammalian

    Available Since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97308

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. attB-­GFP-­Poly(A)

    Plasmid

    #65524

    Purpose

    Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-GFP
    

    Use

    Mouse Targeting; Bxb1

    Expression

    Mammalian

    Available Since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97311

    Purpose

    MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb MMEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. FRB-polySH33-EGFP

    Plasmid

    #175792

    Purpose

    Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97312

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    Lentiviral and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBSc polyA + Shal1

    Plasmid

    #16196

    Depositor

    Larry Salkoff

    Article

    Wei et al Science. 1990 May 4. 248(4955):599-603.

    Insert

    Drosophila melanogaster Shaker cognate l (Shal Fly)
    

    Use

    Xenopus oocyte expression

    Mutation

    N/A

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only