166,041 results

1. pLJM1 Raptor GFP

   Plasmid

   #112744

   Purpose

   express Raptor-GFP

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Raptor-GFP (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 HA E2F1

   Plasmid

   #236431

   Purpose

   transient overexpression of E2F1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

   Insert

   E2F1 (E2F1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-TDP-43 (NLSmut)

   Plasmid

   #235489

   Purpose

   Expression of human TDP-43-EGFP with mutated nuclear localization sequence

   Depositor

   Maxime Rousseaux

   Article

   Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

   Insert

   TARDBP (TARDBP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K82A, R83A, K84A, K95A, K97A, R98A

   Available Since

   May 19, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. NW9

   Plasmid

   #133442

   Purpose

   Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW9
   

   Expression

   Bacterial

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Gamillus/ pcDNA3

   Plasmid

   #124837

   Purpose

   mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

   Insert

   Gamillus
   

   Tags

   No tag

   Expression

   Mammalian

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLG012 (DRT type 4)

   Plasmid

   #157890

   Purpose

   Expresses the DRT type 4 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   DRT type 4
   

   Expression

   Bacterial

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLG013 (DRT type 5)

   Plasmid

   #157891

   Purpose

   Expresses the DRT type 5 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   DRT type 5
   

   Expression

   Bacterial

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTmpl-miniDonor-GFP

   Plasmid

   #238107

   Purpose

   Mini donor expressing GFP, used for PCR to generate IVT template.

   Depositor

   Feng Zhang

   Article

   Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

   Insert

   CMV-GFP-syntheticPA flanked by R2Tg UTRs and 28S sequence
   

   Expression

   Mammalian

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Hygro EYFP H148Q/I152L

   Plasmid

   #25874

   Depositor

   Peter Haggie

   Article

   Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

   Insert

   EYFP H148Q/I152L
   

   Expression

   Mammalian

   Mutation

   EYFP from pEYFP (Clontech) with H148Q and I152L m…

   Available Since

   Aug. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-GGamma10-GFP2

    Plasmid

    #166779

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma10-GFP2 (GNG10 Synthetic, Human)
    

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB_iETV2_P2A_GFP_Puro

    Plasmid

    #168805

    Purpose

    PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ETV2 (ETV2 Synthetic, Human)
    

    Use

    Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. ABE8e-Cas9NG

    Plasmid

    #203328

    Purpose

    Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    ABE8e-Cas9NG
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-DFCP1

    Plasmid

    #86746

    Purpose

    N-terminal mCherry-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human DFCP1 (ZFYVE1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-VSVG

    Plasmid

    #35616

    Depositor

    Arthur Nienhuis, Patrick Salmon

    Insert

    VSV G protein
    

    Expression

    Mammalian

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMV_AA050

    Plasmid

    #216107

    Purpose

    gDNA expression for Cas9 with ChenF+E trRNA (guide only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    gDNA expression for Cas9 with ChenF+E trRNA
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR-A-HYG

    Plasmid

    #29635

    Depositor

    David_L Andrews, Scott Gold

    Article

    Garcia-Pedrajas et al Fungal Genet Biol. 2008 Apr . 45(4):379-

    Type

    Empty backbone

    Use

    Gateway donor

    Available Since

    Oct. 5, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. VPS13D^Halo

    Plasmid

    #174108

    Purpose

    The insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    With an internal Halo tag in aminoacid position 1…

    Promoter

    CMV

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAK005

    Plasmid

    #233642

    Purpose

    For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.

    Depositor

    Zak Swartz

    Article

    Clarke et al Development. 2024 Oct 15;151(20):dev2028

    Insert

    eSpCas9(1.1) with c-terminal VitelloTagB
    

    Tags

    6-His

    Expression

    Mammalian

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNK5785

    Plasmid

    #219753

    Purpose

    pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - nnLuz_v4 - tAOX
    

    Use

    Luciferase

    Expression

    Yeast

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

    Plasmid

    #188777

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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