We narrowed to 1,242 results for: Ott

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pHACK-GAL4>QF2(newHA1)

Plasmid

#104873

Purpose

HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

Depositor

Christopher Potter

Publication

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

T2A-QF2

Use

CRISPR

Expression

Insect

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPEPZ-sgRNAclone

Plasmid

#141090

Purpose

This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.

Depositor

Jan-Willem Veening

Publication

Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

Insert

mCherry

Use

CRISPR

Expression

Bacterial

Promoter

p3

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AR-V7-pcw107-V5

Plasmid

#64636

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Tags

V5

Mutation

splice isoform *

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pHAGE2-EF1aL-mCherry-W

Plasmid

#126703

Purpose

Constitutive, ubiquitous mCherry expression.

Depositor

Darrell Kotton

Publication

McCauley et al Cell Stem Cell. 2017 Jun 1;20(6):844-857

Insert

mCherry

Use

Lentiviral

Promoter

Ef1aL

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-flex-GFP-4x6T

Plasmid

#196418

Purpose

Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Publication

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

GFP

Use

AAV and Cre/Lox; Astrocyte-selective

Expression

Mammalian

Promoter

CAG

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p10xQUAS-CsChrimson

Plasmid

#163629

Purpose

10xQUAS effector expressing CsChrimson.mVenus for red-shifted optogenetic activation of neurons; Contains both P-element ends and attB integration sequence

Depositor

Christopher Potter

Publication

Task et al Elife. 2022 Apr 20;11. pii: 72599. doi:

Insert

CsChrimson.mVenus

Tags

mVenus

Expression

Insect

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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VAMP7 HA-delta pMEP4

Plasmid

#62958

Purpose

full length human VAMP7 with C-terminal HA tag cloned into delta pMEP4

Depositor

Paul Luzio

Publication

Pryor et al Cell. 2008 Sep 5;134(5):817-27. doi: 10.

Insert

VAMP7 (VAMP7 Human)

Tags

HA

Expression

Mammalian

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pNS38-SadCas9-SNAP

Plasmid

#113718

Purpose

Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

Depositor

Gerald Schwank

Publication

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

Cas9

Use

CRISPR

Tags

HA-2xNLS-SNAP-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

D10A, N580A

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBAC-ECFP-15xQUAS_TATA-SV40

Plasmid

#104875

Purpose

piggyBac vector for cloning 15xQUAS transgenes; used for mosquito tranformation

Depositor

Christopher Potter

Publication

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Type

Empty backbone

Expression

Insect

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHAGE-CMV-hAAT-W

Plasmid

#126704

Purpose

Constituitive, ubiquitious human alpha - 1 antitrypsin gene expression

Depositor

Darrell Kotton

Publication

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

hAAT (SERPINA1 Human)

Use

Lentiviral

Promoter

CMVp

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-Arch-GFP

Plasmid

#37810

Depositor

Edward Boyden

Publication

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pHACK-GAL4>GAL80

Plasmid

#104874

Purpose

HACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.

Depositor

Christopher Potter

Insert

T2A-GAL80

Use

CRISPR

Expression

Insect

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-CAHS3

Plasmid

#90031

Purpose

To express GFP-tagged CAHS3 in mammalian cells transiently

Depositor

Takekazu Kunieda

Publication

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CAHS3

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p38 WT O/E (MAPK14)-pcw107-V5

Plasmid

#64624

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAPK14 (transcript variant 2) (MAPK14 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pDONR1K18ms

Plasmid

#72644

Purpose

Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

Depositor

Brian Kvitko

Publication

Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available since

March 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pHAGE2-RSV-jGCaMP7s-W

Plasmid

#126690

Purpose

Mammalian calcium sensor protein.

Depositor

Darrell Kotton

Insert

jGCaMP7s

Use

Lentiviral

Tags

6xHIS, T7, Xpress

Mutation

A52V, A317L

Promoter

RSV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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TGMP.shKras.1422

Plasmid

#59913

Purpose

Tet on shKras for conditional Kras knockdown

Depositor

Tyler Jacks

Publication

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

Kras (Kras Mouse)

Use

Retroviral

Available since

Dec. 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK1036

Plasmid

#17728

Depositor

David Waugh

Publication

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

NusG (nusG Aquifex aeolicus)

Tags

His6 and MBP

Expression

Bacterial

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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Luciferase-pcw107

Plasmid

#64648

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Promoter

PGK

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
6xHis-MBP-BrCas12b

Plasmid

#182276

Purpose

Expresses recombinant BrCas12b

Depositor

Piyush Jain

Publication

Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis, T7, Thrombin site

Expression

Bacterial

Promoter

T7

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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