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pSaI14

Plasmid

#8614

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI14

Use

Tags

Expression

Bacterial

Mutation

N terminal threonine changed to alanine

Promoter

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pUAS-kkv-mos::NG

Plasmid

#138958

Purpose

Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4

Depositor

Paul Adler

Article

Adler et al bioRxiv 2020

Insert

kkv (NEWENTRY Fly)

Use

Uas transgene vector

Tags

Neon Green fused to C terminus of Kkv

Expression

Insect

Mutation

8 bp that are in the region that encodes the cata…

Promoter

UAS

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAcGFP-Jpx9

Plasmid

#120379

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

JPX (JPX Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 nt 400-1782 delta440-500 delta1449-1735

Plasmid

#60633

Purpose

ZKSCAN1 circular RNA expression plasmid containing minimal sufficient introns

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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C238-E02: attB2r-BGHpolyA-attB3

Plasmid

#162916

Purpose

Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Bovine growth hormone polyadenylation sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGC4780

Plasmid

#113408

Purpose

Translation reporter with inducible translation coupling (high native GFP expression)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Insert

158_33121123_1

Use

Synthetic Biology

Tags

fused to codon deptimized sfGFP with Flexible lin…

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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Mouse NHERF-1

Plasmid

#32950

Depositor

Edward Weinman

Article

Weinman et al Biochim Biophys Acta. 1999 Oct 6;1447(1)

Insert

Slc9a3R1 (Nherf1 Mouse)

Use

Tags

none

Expression

Bacterial

Mutation

none

Promoter

t3

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pHu6-gRNA-NT1

Plasmid

#81203

Purpose

hU6 expression of gRNA NT1

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA NT1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pAcGFP-Jpx9C104A

Plasmid

#120380

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

JPX (JPX Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

C321A

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-mOrange pHis-//

Plasmid

#53302

Purpose

Expresses His-tagged EGFP-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

EGFP

Use

Tags

His and mOrange

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pSaI20

Plasmid

#8615

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI20

Use

Tags

Expression

Bacterial

Mutation

N terminal threnine changed to alanine

Promoter

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-ZKSCAN1 nt 400-1782-SV40 pA Sense

Plasmid

#60643

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pPTK007-3a-αMFΔ

Plasmid

#84966

Purpose

Type 3a, α-mating factor pre-sequence shortened - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor, pre-sequence shortened secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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GST-Smad4-MH1

Plasmid

#16492

Depositor

Bert Vogelstein

Article

Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

Insert

Smad4 (SMAD4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-276 of Smad4

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pKJ-1

Plasmid

#11333

Depositor

Michael McBurney

Article

Adra et al Gene. 1987 . 60(1):65-74.

Insert

pgk-1 5' and 3' regions (Pgk1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2006

Availability

Academic Institutions and Nonprofits only
PJ14

Plasmid

#25912

Depositor

Kenneth Marcu

Article

Lang et al Nucleic Acids Res. 1982 Jan 22. 10(2):61

Insert

Ig germline mu switch region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only
pGL3con-miR15aBul

Plasmid

#21117

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-15a sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_PGKY_APikL3

Plasmid

#123652

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

PGKY_APikL3

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_CD156_APikL3

Plasmid

#123651

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

CD156_APikL3

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_BR32_APikL2

Plasmid

#123649

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

BR32_APikL2

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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