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pTC223

Plasmid

#70019

Purpose

Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (Cas9/sgRNA) + Donor + GVR

Use

CRISPR

Expression

Plant

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-C1-P4Mx2

Plasmid

#202681

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

PAmCherry1: L. pneumophila SidM(546-647):SidM(546-647) (sidM Legionella pneumophila)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)

Plasmid

#192123

Purpose

Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)

Depositor

Wilson Wong

Article

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry

Use

Lentiviral

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-1

Plasmid

#61736

Purpose

Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

Depositor

Huimin Zhao

Article

Cobb et al ACS Synth Biol. 2014 Dec 8.

Inserts

sSpCas9
tracrRNA
crRNA cassette

Use

CRISPR

Expression

Bacterial

Mutation

BbsI-flanked lacZ cassette inserted in place of s…

Promoter

gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD_TypeIC_Cascade_cas3_invivo

Plasmid

#196400

Purpose

expresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Inserts

Cas5c
Cas7c
Cas8c
Cas3c

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCC2)

Plasmid

#235072

Purpose

Expresses Tad-CBE(CCC2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCC2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY-Tad-CBE(ACC2)

Plasmid

#235081

Purpose

Expresses SpRY-Tad-CBE(ACC2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACC2)-SpRY Cas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPAmCherry3-C1

Plasmid

#31933

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry3

Expression

Mammalian

Mutation

M18L/A58T/K69N/L84F/M137I/E146D/S148L/I165L/Q167A…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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MBP-CRY1-PHR

Plasmid

#26035

Depositor

Johann Deisenhofer

Article

Brautigam et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

CRY1 (CRY1 Mustard Weed)

Tags

MBP

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pDGE335

Plasmid

#153244

Purpose

M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-SsAPOBEC3B

Plasmid

#138343

Purpose

Mammalian expression plasmid for BE4-SsAPOBEC3B

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-SsAPOBEC3B

Use

Synthetic Biology

Tags

BP-NLS

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTlpA_mCherry_YafQ/DinJ

Plasmid

#229148

Purpose

Toxin-Antitoxin (Dinj/YafQ)

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry, DinJ/YafQ

Expression

Bacterial

Promoter

PtlpA (mCherry), DinJ/YafQ (Native)

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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TurboRFP-pBAD

Plasmid

#54858

Purpose

Localization: Protein Expression Vector , Excitation: 553, Emission: 574

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Tags

6xHis and TurboRFP

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAJM.1642

Plasmid

#108535

Purpose

CinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

CinRAM + PCin-YFP

Expression

Bacterial

Mutation

CinR: S72C V80I G114D G115D R137H K220R A224T *24…

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pOVC1_ScPH1-S_X

Plasmid

#131184

Purpose

Expresses ScPH1-S (Synechosystis PCC6803) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

ScPH1-S

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pJP_Osc05

Plasmid

#229040

Purpose

Light inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary.

Depositor

Yolanda Schaerli

Article

Hollo et al Mol Syst Biol. 2025 Sep 29. doi: 10.1038

Insert

pBAD promoter

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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DR274-eGFP sgRNA

Plasmid

#61051

Purpose

for generation of a eGFP specific sgRNA from a T7 promoter

Depositor

Filippo Del Bene

Article

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Insert

EGFP sgRNA

Use

CRISPR

Promoter

T7

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-C1-P4C

Plasmid

#202680

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

PAmCherry1: L. pneumophila SidC (608-773) (sidC Legionella pneumophila)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_PFN1_Profilin

Plasmid

#110028

Purpose

Protein expression and purification of PFN1_Profilin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PFN1_Profilin (PFN1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(TCT1)

Plasmid

#235065

Purpose

Expresses Tad-CBE(TCT1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCT1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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