We narrowed to 92,119 results for: lias

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pHIE058 ZF43x0 mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138739

Purpose

Integration Vector, assembled with pHIE042 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x0

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P04

Plasmid

#120015

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/PH* (W495L) uGFP

Plasmid

#14564

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

PH* (W495L)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
BII-C3HTK

Plasmid

#133388

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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MTK2_024

Plasmid

#123716

Purpose

Encodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pPGK1c

Expression

Mammalian

Mutation

Crippled Kozac sequence

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)

Plasmid

#139230

Purpose

mMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTS2404-Tier1-PhCMV-NeoR

Plasmid

#169496

Purpose

Tier-1 vector encoding PhCMV-driven neomycin resistance gene NeoR (PhCMV-NeoR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven aminoglycoside 3'-phosphotransferase, APH 3' II

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/CR* (C529S) uGFP

Plasmid

#14560

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/CR* (C529S)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.1 EB1 shRNA #1

Plasmid

#37928

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 shRNA #1 (MAPRE1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

human U6

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pFOX13-Tier1-PhCMV-tGFP

Plasmid

#169510

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein turbo GFP (PhCMV-tGFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven turbo green fluorescent protein

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-LDR

Plasmid

#173857

Purpose

Encoding a part of the rapamycin-induced dimerization system.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

a Lyn11-targeted FRB (LDR)

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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4xHRE_MLP-sfGFP-CMV_dsRed Exp

Plasmid

#117403

Purpose

sfGFP expressed from hypoxia-inducible MLP promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pNTI757 P(PGK1)-citrine P(HIS4)-mCherry

Plasmid

#164917

Purpose

Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(PGK1)-citrine and P(HIS4)-mCherry

Expression

Yeast

Promoter

PGK1 (yeast) and HIS4 (yeast)

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-NLS-3XHA-NLS

Plasmid

#69220

Purpose

Expresses wild type SpCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Syn3D33A

Plasmid

#13400

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

Myc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector. D33 of s…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

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pFUPI

Plasmid

#80279

Purpose

A bicistronic lentiviral vector used to stably express cDNAs.

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

Puro resistance cassette, IRES, cloning site

Use

Lentiviral

Expression

Mammalian

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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Syn3E34A

Plasmid

#13401

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

Myc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector. E34 of s…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pBS-BtH

Plasmid

#133392

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLX-V5-TRAF2 S11A-puro

Plasmid

#44112

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S11A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Mutation

Serine 11 to Alanine

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only
pTK_Cerulean_Nanotag_66_neg_Control

Plasmid

#130567

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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