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Plasmid#208032PurposeEnables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only
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pSlick-Neo-RECK
Plasmid#121986PurposeTetracycline-inducible expression of untagged full-length RECKDepositorInsertRECK (RECK Human)
UseLentiviralTagsExpressionMutationPromoterTight TRE promoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-UBC-DSRED-LUC-EF1 Hygro
Plasmid#129437Purposeexpress dsred-luc chimeraDepositorInsertdsred-luc
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
HDM-Hgpm2
Plasmid#204152PurposeLentivirus helper plasmid coding for Gag/PolDepositorInsertHIV-1 gag/pol
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Donor_ANKRD1_KO_Puro
Plasmid#186667PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Puromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiV2-dCas9-VP64
Plasmid#141104PurposeExpression of gRNA and dCas9-VP64 from the same plasmid for CRISPRaDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_mScarlet-I_Donor
Plasmid#173207PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N mScarlet-I donor
UseCRISPRTagsExpressionMammalianMutationPromoterhPGK1Available sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
R777-E211 Hs.RAC3
Plasmid#70495PurposeGateway ORF clone of human RAC3 [NM_005052.2] with stop codon (for native or N-terminal fusions)DepositorInsertRAC3 (RAC3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 GFPNanobody_SNAP
Plasmid#82711PurposeExpresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.DepositorInsertGFP Nanobody
UseTags6xHis Purification tag, FLAG purification tag, an…ExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR V5-RNF25_IRES-mCherry
Plasmid#198386PurposeRNF25 expressionDepositorInsertRNF25 (RNF25 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCsRNAP
Plasmid#182746PurposeSwapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmidsDepositorInsertsmall RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PSD-95-pTagRFP
Plasmid#52671Purposeexpression of TagRFP-tagged PSD-95DepositorInsertPSD-95 (Dlg4 Rat)
UseTagstagRFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pM355
Plasmid#173175Purpose(Empty Backbone) Expresses gRNA-12xMBS from hU6 promoterDepositorInsertgRNA-12xMBS backbone
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF1538
Plasmid#143823PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZBTB46 (ZBTB46 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-Ef1a-fDIO-mCherryP2ACre
Plasmid#161773PurposeFlp-Dependent mCherry and Cre expressionDepositorInsertmCherry-P2A-Cre
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDG_GABRP_OE_1
Plasmid#161679PurposeOverexpresses GABRPDepositorInsertGABRP (GABRP Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAP2K1
Plasmid#23406DepositorInsertMAP2K1 (MAP2K1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 DPP9S
Plasmid#166789PurposepDONR gateway shuttling vector with DPP9S (no stop codon)DepositorInsertDPP9 (DPP9 Human)
UseGateway shuttling vectorTagsExpressionMutationPromoterAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only