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pH2B-mScarlet3-H (aka mYongHong)

Plasmid

#239404

Purpose

Expresses H2B-mScarlet3-H in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

H2B (H2BC21 Human)

Tags

mScarlet3-H (aka mYongHong)

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAXY0002

Plasmid

#179835

Purpose

Binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

Depositor

Xiaohan Yang

Article

Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

Type

Empty backbone

Use

Binary vector for plant transformation

Expression

Plant

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAGE

Plasmid

#71435

Purpose

mammalian expression of human RAGE

Depositor

Henri Huttunen

Article

Huttunen et al J Biol Chem. 1999 Jul 9;274(28):19919-24

Insert

RAGE (AGER Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pBOBI-TRPV2-C-GC6s

Plasmid

#249436

Purpose

Used for transfection of cells for expression of TRPV2 and GCamp6s

Depositor

Sheng-cai Lin

Article

Sun et al Nat Metab. 2025 Oct 22. doi: 10.1038/s42

Insert

TRPV2 and GCamp6s (TRPV2 Human)

Expression

Mammalian

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pRS426-HsEMRE

Plasmid

#253497

Purpose

Expresses HsEMRE in pACT2 vector

Depositor

Vamsi Mootha

Article

Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

Hs EMRE (SMDT1 Human)

Expression

Bacterial and Yeast

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1alpha-DEST-IRES-Neo

Plasmid

#253766

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pACT2-DdtS-HsMCU

Plasmid

#253519

Purpose

D.discoideum targeting sequence with C-terminal tagged HsMCU mature protein in pACT2 vector

Depositor

Vamsi Mootha

Article

Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

HsMCU (MCU Human)

Tags

FLAG

Expression

Bacterial and Yeast

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLYS1- DdMCU_AIIA, Cterm Flag

Plasmid

#253538

Purpose

Expresses C-terminal Flag tagged DdMCU_AIIA

Depositor

Vamsi Mootha

Article

Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

DdMCU (DDB_G0286429 Dictyostelium discoideum)

Use

Lentiviral

Tags

Flag

Mutation

changed D192A and E195

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pVAM07

Plasmid

#252057

Purpose

Hygromycin B resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Article

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pVAM05

Plasmid

#252056

Purpose

Gentamicin resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Article

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pVAM03

Plasmid

#251701

Purpose

Tetracycline resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Article

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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AiP1568 - pAAV-AiE2235m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#241537

Purpose

AiE2235m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pINEAPLE

Plasmid

#250295

Purpose

Big-IN Payload assembly vector by Golden Gate Cloning using Esp3I. Contains a bacterial green/white GFP-based screening system and a mammalian transient (backbone) BSD selection cassette.

Depositor

Matthew Maurano

Article

Ordoñez et al bioRxiv 2025.08.13.669251

Insert

SuperfolderGFP

Use

Synthetic Biology; Assembly vector for big-in pay…

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pB-Ef1a-HERVK-proteins

Plasmid

#248162

Purpose

Expresses HERVK proteins and tagBFP

Depositor

Joanna Wysocka

Article

Fueyo et al Nature. 2025 Oct 1. doi: 10.1038/s41586-

Inserts

HERVK GAG-PRO-POL
tagBFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#242162

Purpose

Lentiviral expression of dCas9-VPR with puromycin reporter

Depositor

Amy Brock

Insert

dCas9-VP64-RelA-Rta; T2A-puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAG AAV CMV Empty

Plasmid

#242957

Purpose

Empty AAV expression backbone

Depositor

Meghan Rego, Addgene Research Program

Type

Empty backbone

Use

AAV

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV hSyn:FLEX-Soma-jGCaMP8s

Plasmid

#239669

Purpose

Cre-dependent expression of Soma-jGCaMP8s under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8s

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8f

Plasmid

#239671

Purpose

Cre-dependent expression of Soma-jGCaMP8f under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8f

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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1162BY-CMV-mCherry-bGHpolyA

Plasmid

#235172

Purpose

Expresses mCherry

Depositor

Omar Akbari

Insert

mCherry

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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AiP20063 - pAAV-AiE3114m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230793

Purpose

AiE3114m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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