We narrowed to 11,714 results for: NSI

1. pcDNA3/hArf6(T27N)-HA

   Plasmid

   #79426

   Purpose

   Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf6 (ARF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed Threonine 27 to Asparagine

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pExp-xMBP-ACE2-CHis

   Plasmid

   #194998

   Purpose

   Production of ACE2 extracellular domain

   Depositor

   Marko Hyvönen

   Article

   Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

   Insert

   ACE2 (ACE2 Human)
   

   Tags

   His8 and xMBP

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3/hArf1(WT)-mCherry

   Plasmid

   #79419

   Purpose

   Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf1 (ARF1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-nEF-Coff/Fon-iC++-EYFP

   Plasmid

   #137157

   Purpose

   Intersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-iC++-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry

   Plasmid

   #231007

   Purpose

   Protein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

   Insert

   E4F1 aa220-242 (E4F1 Human)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3/hArf6(WT)-mCherry

   Plasmid

   #79422

   Purpose

   Expresses C-terminally mCherry-tagged Arf6(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf6 (ARF6 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBT272_(pRosa26-GTET)

   Plasmid

   #36882

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tTA2
   beta-globin intron
   Neo
   tdT-3Myc
   insulator
   diphteria toxin A
   

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available Since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. Synapsin sHRPb-NLG in FSW lentiviral vector

   Plasmid

   #73148

   Purpose

   Small fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPb-NLG (Nlgn1 Mouse, Synthetic)
   

   Use

   Lentiviral

   Tags

   V5 tag

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXS-IRES-BLAST TFR1

   Plasmid

   #169890

   Purpose

   Express TFR1 open reading frame. Lacks IRE regulatory elements

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   TFRC (TFRC Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   TFRC cDNA, lacking 3'UTR regulatory elements

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223 SARS-CoV-2 ORF3B

    Plasmid

    #141272

    Purpose

    Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF3B
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3/hArf3(WT)-mCherry

    Plasmid

    #79420

    Purpose

    Expresses C-terminally mCherry-tagged Arf3(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf3 (ARF3 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid

    #118586

    Purpose

    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

    Depositor

    Radhakrishnan Padmanabhan

    Article

    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

    Insert

    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    

    Use

    Yeast-e. col shuttle vector prs424

    Mutation

    The conserved GDD in the NS5 polymerase gene muta…

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223-ZNF846

    Plasmid

    #88355

    Purpose

    Donor Vector containing ZNF846 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF846 (ZNF846 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC1GFP-Kir7.1

    Plasmid

    #79118

    Purpose

    Express N-terminal fused Kir7.1 protein in mammalian cells

    Depositor

    Bikash Pattnaik

    Article

    Pattnaik et al PLoS One. 2013 Aug 19;8(8):e71744. doi:

    Insert

    Inwardly rectifying potassium channel KCNJ13 (KCNJ13 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-nEF-Con/Foff 2.0-iC++-EYFP

    Plasmid

    #137156

    Purpose

    Intersectional viral expression of ic++-EYFP in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-iC++-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    n/a

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEos3.2FPP

    Plasmid

    #203758

    Purpose

    Encodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    HRas c-term (HRAS Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRM049 His-TEV-Cas9 triNLS 2C

    Plasmid

    #196245

    Purpose

    Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3xFLAG CMV14 / BRAF-D594G

    Plasmid

    #131719

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D594G

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGLS3-5xHRE-p53(K120R)

    Plasmid

    #72554

    Purpose

    Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    p53 (TP53 Human)
    

    Tags

    5XHRE

    Expression

    Mammalian

    Mutation

    K120R

    Promoter

    E1B minimal promoter

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI-neo-RL-ZEB1 200bmutx5

    Plasmid

    #35537

    Depositor

    Greg Goodall

    Article

    Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

    Insert

    3'UTR of ZEB1 (ZEB1 Budding Yeast, Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    5 mutations in miR200b seed regions of 3'UTR…

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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