We narrowed to 9,182 results for: Pol

1. pLIB-RSP12

   Plasmid

   #176700

   Purpose

   Expression of Chlamydomonas reinhardtii RSP12 recombinant protein in insect cells

   Depositor

   Ron Vale

   Article

   Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

   Insert

   RSP12
   

   Expression

   Insect

   Mutation

   None

   Promoter

   polyhedrin

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLIB-RSP16

   Plasmid

   #176701

   Purpose

   Expression of Chlamydomonas reinhardtii RSP16 recombinant protein in insect cells

   Depositor

   Ron Vale

   Article

   Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

   Insert

   RSP16
   

   Expression

   Insect

   Mutation

   None

   Promoter

   polyhedrin

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIB-RSP23

   Plasmid

   #176702

   Purpose

   Expression of Chlamydomonas reinhardtii RSP23 recombinant protein in insect cells

   Depositor

   Ron Vale

   Article

   Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

   Insert

   RSP23
   

   Expression

   Insect

   Mutation

   None

   Promoter

   polyhedrin

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK793

   Plasmid

   #8827

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   TEV protease, S219V mutant
   

   Tags

   His, MBP (with TEV), and polyarginine

   Expression

   Bacterial

   Mutation

   S219V mutation (improves stability)

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHM6-Q23

   Plasmid

   #40263

   Depositor

   David Rubinsztein

   Article

   Narain et al J Med Genet. 1999 Oct;36(10):739-46.

   Insert

   HTT partial exon 1 Q23 (HTT Human)
   

   Tags

   6x His and HA

   Expression

   Mammalian

   Mutation

   expanded poly-Q

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHM6-Q74

   Plasmid

   #40264

   Depositor

   David Rubinsztein

   Article

   Narain et al J Med Genet. 1999 Oct;36(10):739-46.

   Insert

   HTT partial exon 1 Q74 (HTT Human)
   

   Tags

   6x His and HA

   Expression

   Mammalian

   Mutation

   expanded poly-Q

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIB

   Plasmid

   #80610

   Purpose

   pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLiLucB

   Plasmid

   #31596

   Depositor

   Michele Calos

   Article

   Woodard et al PLoS One. 2010 . 5(6):e11367.

   Insert

   alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. HPRT

   Plasmid

   #22884

   Depositor

   James Thomson

   Article

   Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

   Insert

   hypoxanthine phosphoribosyltransferase-1 (HPRT1 Human)
   

   Use

   Cre/Lox; Homologous recombination

   Mutation

   PGK, NeoR, and BGH polyA are between the two geno…

   Available Since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-Q74

    Plasmid

    #40262

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCJ1021

    Plasmid

    #228761

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 gRNAs (Ift88 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-Q23

    Plasmid

    #40261

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q23 (HTT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. c-Flag pcDNA3

    Plasmid

    #20011

    Purpose

    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEHlyA5

    Plasmid

    #168298

    Purpose

    Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

    Depositor

    Luis Ángel Fernández

    Article

    Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

    Insert

    N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
    

    Tags

    E-tag, HA-tag, His-tag, Prescission site, and VHH…

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. LEGO-pDHS-ITDE-AP1x6-DUSP5

    Plasmid

    #217419

    Purpose

    Luciferase expression vector with an oncogene-inducible enhancer, 6 AP-1 sites, the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6-ITDe

    Depositor

    Peter Cockerill

    Insert

    Luciferase, GFP
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    Human DUSP5 promoter

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFB1_hMSH6

    Plasmid

    #129424

    Purpose

    Expression human MSH6 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH6 (MSH6 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN7CAST

    Plasmid

    #190661

    Purpose

    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFB1_hMSH2

    Plasmid

    #129423

    Purpose

    Expression human MSH2 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH2 (MSH2 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSUPERretro-Sirt7 shRNA2

    Plasmid

    #53145

    Purpose

    shRNAi vector for Sirt7

    Depositor

    Katrin Chua

    Article

    Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

    Insert

    Sirt7
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    H1 RNA polymerase III

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only