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XW82

Plasmid

#65840

Purpose

MosSCI-QUAS::Δpes10::GFP

Depositor

Kang Shen

Publication

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

5XQUAS-delta-pes-10::GFP

Tags

GFP

Expression

Worm

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pNAS1B split mCherry N-MESVD/TRAP(RK)-C

Plasmid

#61967

Purpose

Duet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD

Depositor

Lynne Regan

Publication

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-MESVD
TRAP(RK)-C-mCherry

Expression

Bacterial

Promoter

PBAD and PLtetO

Available since

Feb. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomApple-CaURA3

Plasmid

#44879

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Expression

Yeast

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS405-FlagPEN2/PS1wt

Plasmid

#207803

Purpose

Yeast integrative vector for expression of FlagPEN2 and Presenilin 1

Depositor

Dieter Langosch

Publication

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits PEN-2 and presenilin1 wt

Expression

Yeast

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-GFP-BsaI_pJMP2748

Plasmid

#208888

Purpose

Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).

Depositor

Jason Peters

Publication

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette, sfGFP

Use

Pir dependent origin of replication

Expression

Bacterial

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRS402-nicastrin/APH-1a-HA

Plasmid

#207804

Purpose

Yeast integrative vector for expression of Nicastrin and APH-1a-HA

Depositor

Dieter Langosch

Publication

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits nicastrin and Aph1a

Expression

Yeast

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-Nucleocapsid

Plasmid

#168906

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-Nucleocapsid (N )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:redCas9; control_gRNA

Plasmid

#199334

Purpose

pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
control sgRNA

Use

Tol2 destination vector

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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PET-3a-chpBK

Plasmid

#155201

Purpose

To express recombinant ChpBK protein

Depositor

Guanzheng Luo

Publication

Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

Inserts

chpS-chpBK-6×His-Tag
GyrA-CBD-10xHis-Tag

Tags

10xHis and 6xHis

Expression

Bacterial

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pQCascade-IS186

Plasmid

#140627

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186

Use

CRISPR; Transposon

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pM9_NR_HD

Plasmid

#90415

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ NR promotor_HD

Depositor

Peter Kroth

Publication

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-sgNeo2/Cre

Plasmid

#89652

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Publication

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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BC-A1-006

Plasmid

#78693

Purpose

A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.

Depositor

Brian Chow

Publication

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator

Use

Synthetic Biology

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A_FokI-1x4

Plasmid

#63604

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Publication

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pX330A_FokI-1x7

Plasmid

#63607

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Publication

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pRVdG-4BFP2-5postmGRASP

Plasmid

#52498

Purpose

Expresses mTagBFP2 and postsynaptic mGRASP component

Depositor

Ian Wickersham

Inserts

mTagBFP2
postsynaptic mGRASP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCS4

Plasmid

#55751

Purpose

Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Publication

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Expression

Bacterial

Mutation

Residues 1-59 of KanR

Promoter

T7-lac

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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porcupine - Xenopus

Plasmid

#39772

Depositor

Norbert Perrimon

Publication

Kadowaki et al Genes Dev. 1996 Dec 15;10(24):3116-28.

Insert

porcupine (porcn Frog)

Expression

Bacterial

Available since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only
pKLBac-FluB-trimer

Plasmid

#229859

Purpose

Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Publication

Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

Inserts

PA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-Cre-MS2_UbC-GFP (Cre_GFP)

Plasmid

#219536

Purpose

a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotor

Depositor

Jeffrey Gerst

Publication

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

Cre recombinase (cre )
GFP

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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