165,343 results

1. dCas12j3 VPR

   Plasmid

   #188517

   Purpose

   Expresses FLAG tagged dCas12j3 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j3
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D413A

   Promoter

   EF1a

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJRK246

   Plasmid

   #173741

   Purpose

   donor plasmid for SapTrap assembly

   Depositor

   Mike Boxem

   Article

   Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

   Insert

   wrt-2 promoter
   

   Use

   Other

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDD373

   Plasmid

   #137021

   Purpose

   SapTrap GFP-GLO donor

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   C. elegans GLO-optimized GFP
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized GFP

   Expression

   Worm

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIVT-R2Tg-WT

   Plasmid

   #238105

   Purpose

   For IVT of wildtype R2Tg editor, codon optimized for mammalian expression.

   Depositor

   Feng Zhang

   Article

   Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

   Insert

   Wildtype R2Tg, mammalian codon optimized
   

   Use

   For ivt

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSYN1-fDIO-tTA

   Plasmid

   #166597

   Purpose

   Encodes Flp-dependent tTA

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   tTA
   

   Use

   AAV

   Promoter

   human synapsin 1

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMLS714

   Plasmid

   #188886

   Purpose

   MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   Pmex-5::Cas9
   

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-TDP-43 (NLSmut)

   Plasmid

   #235489

   Purpose

   Expression of human TDP-43-EGFP with mutated nuclear localization sequence

   Depositor

   Maxime Rousseaux

   Article

   Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

   Insert

   TARDBP (TARDBP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K82A, R83A, K84A, K95A, K97A, R98A

   Available Since

   May 19, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. HIV-1-GFP ΔEnv

   Plasmid

   #217437

   Purpose

   HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   gag/pol
   

   Use

   Lentiviral

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_hSyn1_nLightG

   Plasmid

   #187179

   Purpose

   Expresses green norepinephrine indicator nLightG in neuronal cells

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Has Service

   AAV9

   Insert

   nLightG
   

   Use

   AAV

   Tags

   Flag tag

   Promoter

   human Synapsin-1

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. dg264_pENTR_ChronosCel

    Plasmid

    #66102

    Purpose

    Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    Chronos
    

    Use

    Gateway

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFSW GFP IRES GFP

    Plasmid

    #133815

    Purpose

    Encodes EGFP that can be used as a control for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-GFP

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid

    #160437

    Purpose

    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Homo sapiens amyloid beta precursor protein (APP Human, Nematode)
    

    Tags

    Cleavage signal

    Expression

    Bacterial

    Mutation

    Codon-optimized for expression in C. elegans

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid

    #183410

    Purpose

    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    KRAB-dCas9-ecDHFR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. 4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

    Plasmid

    #174529

    Purpose

    Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Inserts

    PylS
    PylT
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUW-tetO-Batf3

    Plasmid

    #139837

    Purpose

    doxycycline-inducible expression of mouse Batf3 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Batf3 (Batf3 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

    Plasmid

    #164611

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Expression

    Mammalian

    Mutation

    S383T

    Promoter

    CMV

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

    Plasmid

    #199349

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-SUMOEu

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-EML4-ALK variant 1

    Plasmid

    #183828

    Purpose

    Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-13 and ALK exon…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWM_12x601_15bpLinker

    Plasmid

    #157786

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_15bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sfp pet29b C-terminal His Tag

    Plasmid

    #75015

    Purpose

    Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

    Depositor

    Michael Burkart

    Article

    Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

    Insert

    Sfp
    

    Tags

    His Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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