173,520 results
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Plasmid#195504PurposeCas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17DepositorInsertsgRNA
UseCRISPRTags3XFLAG, Puro, and T2AExpressionMammalianPromoterU6Available SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT
Plasmid#184774PurposeMarker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.DepositorAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP214-Lenti-0.5Synapsin P-GFP- WPRE-pA
Plasmid#62929PurposepLenti vector backbone designed to express GFP from a 0.5Synapsin promoter.DepositorInsertGreen Fluorescent protein (GFP)
UseLentiviralPromoter0.5synapsin and 1.3αCaMKIIAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mRuby3-Zeo
Plasmid#167206PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with ZeocinDepositorTypeEmpty backboneUseCRISPRTagsmRuby3ExpressionMammalianAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE EF1α dCas9-KRAB
Plasmid#50919PurposeConstitutive dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840APromoterEF1alphaAvailable SinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-TAZ
Plasmid#32839DepositorAvailable SinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg1
Plasmid#127158PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotypeDepositorAvailable SinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-EYFP
Plasmid#26969PurposeAAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activationDepositorHas ServiceAAV1, AAV2, AAV5, and AAV9InserthChR2(H134R)
UseAAVTagsEYFPExpressionMammalianMutationHistidine 134 is mutated to ArgininePromoterCaMKIIaAvailable SinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 BRGI-Flag
Plasmid#19143DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
TagsFlagExpressionMammalianAvailable SinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RN
Plasmid#60519PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
JL054: pPB_NFκB::GFP-GEMINI(A)-P1N4_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR
Plasmid#228884PurposePiggyBac plasmid expressing the destabilized GFP-GEMINI(A) under the control of a synthetic NFκB-responsive promotor, and rtTA3 and PuroR under the control of a UbC promoter.DepositorInsertGFP-fused GEMINI(A), HaloTag-fuse GEMINI(A)
ExpressionMammalianPromoterpNFκB; TREAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLX303
Plasmid#25897Purpose3rd generation lentiviral Gateway destination vector. Blasticidin selection.DepositorTypeEmpty backboneUseGateway Cloning and LentiviralExpressionMammalianAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
GALT-SEP-mRuby3
Plasmid#200938Purposetrans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
ampkar
Plasmid#35097PurposeA fluorescent reporter of AMPK activity and cellular energy stressDepositorInsertampkar
TagsECFP and VenusExpressionBacterialMutationnaPromoterCMVAvailable SinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-active WRN
Plasmid#125788Purposeconstitutive, ectopic expression of human active WRNDepositorAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2-HuC(elavl3)-CaMPARI2
Plasmid#137185Purposepan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)DepositorInsertHuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
UseTol2 plasmid for zebrafishTagsFLAG, HA, mycPromoterHuC(elavl3)Available SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH0006
Plasmid#135448PurposeHuman expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.DepositorInsertdCas9-BFP-KRAB
UseLentiviralTagstagBFPExpressionMammalianPromoterEF1AAvailable SinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-p105
Plasmid#23288DepositorInsertp105 (NFKB1 Human)
ExpressionMammalianAvailable SinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flpo
Plasmid#55637PurposeExpresses Flpo in Mammalian CellsDepositorHas ServiceAAV Retrograde, AAV1, and AAV2InsertFlpo
UseAAVExpressionMammalianPromoterEf1aAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only