162,590 results

1. HRV-B3 IRES

   Plasmid

   #189969

   Purpose

   Part 2 plasmid to introduce the HRV-B3 IRES into circRNAs

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   HRV-B3 IRES
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-Brainbow-2.1 R

   Plasmid

   #18723

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM1-Tmem192-mRFP-3xHA

   Plasmid

   #134631

   Purpose

   bait for affinity-isolation of lysosomes

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   3xHA, TEV, and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE

   Plasmid

   #198516

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVi U6_gRNA CMV_SadCas9-KRAB

   Plasmid

   #214609

   Purpose

   All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

   Depositor

   Ralf Gilsbach

   Article

   Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

   Inserts

   gRNA
   SadCas9
   

   Use

   AAV

   Tags

   NP NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   CMV and U6

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-polyQ23-NLS

   Plasmid

   #231891

   Purpose

   Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q23-NLS (HTT Human)
   

   Use

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. A547 (miR-146a promoter)

   Plasmid

   #15091

   Depositor

   David Baltimore

   Article

   Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

   Insert

   miR-146a promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-GRAB_r5-HT1.0

   Plasmid

   #208719

   Purpose

   Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Red fluorescent 5-HT sensor GRAB_r5-HT1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. SP1-SB100X

   Plasmid

   #154887

   Purpose

   Sleeping Beauty transposase fused to SP1

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   SP1-Sleeping Beauty
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pITGA4-GFP

    Plasmid

    #81178

    Purpose

    Mammalian expression of human full length ITGA4-EGFP

    Depositor

    Chinten James Lim

    Insert

    ITGA4 (ITGA4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains KLGFFKR motif; A1002L (please see deposi…

    Promoter

    CMV

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVCT-tTR-KRAB

    Plasmid

    #11643

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    CAG promoters, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. MZB209

    Plasmid

    #228492

    Purpose

    BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)

    Depositor

    Richard Baker

    Insert

    BirA (birA )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

    Plasmid

    #229795

    Purpose

    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-hATG7wt

    Plasmid

    #87867

    Purpose

    Express a wild type human Atg7/Apg7 in mammalian cells

    Depositor

    Eiki Kominami, Isei Tanida

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    ATG7 (ATG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. mTagRFP-T-Golgi-7

    Plasmid

    #58018

    Purpose

    Localization: Golgi Complex, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

    Plasmid

    #52092

    Purpose

    Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

    Inserts

    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    

    Use

    Retroviral

    Tags

    F2A, IRES-EGFP, P2A, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 (-) hYAP 1-2alpha

    Plasmid

    #124143

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-6xHis-(-30)GFP

    Plasmid

    #62936

    Purpose

    Expression of 6xHis-(-30)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-WHaloCaMP1a-EGFP

    Plasmid

    #205313

    Purpose

    Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. psiCHECK2-let-7 MT

    Plasmid

    #78261

    Purpose

    psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    mutated let-7 target site
    

    Use

    Luciferase; Microrna activity

    Tags

    Expression

    Mammalian

    Mutation

    nucleotides 2-5 and 10-11 changed from CTCC to GA…

    Promoter

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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