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pmyc-GFP-TNRC6A-(925-1709)

Plasmid

#42004

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa925-1709

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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TFORF1108

Plasmid

#143254

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CREBBP (CREBBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iGABASnFR

Plasmid

#112159

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has Service

AAV1

Insert

iGABASnFR

Use

AAV

Tags

Expression

Mutation

Promoter

hSynapsin

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS

Plasmid

#162610

Purpose

Allows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Use

Tags

Expression

Mammalian

Mutation

Contains aa1-155 of mVenus

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Article

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Msx1

Plasmid

#84313

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Msx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Msx1 (Msx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-Homer1-N-18

Plasmid

#54121

Purpose

Localization: Membrane Adaptor, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Homer1 (Homer1 Rat)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pmyc-GFP-TNRC6A-(1-924)

Plasmid

#42003

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa1-924

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-RAB10

Plasmid

#130883

Purpose

Expresses human RAB10 with N-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGCaMP6m-N3-TPC2

Plasmid

#80147

Purpose

GCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real time

Depositor

Santiago Di Pietro

Article

Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7

Insert

TPCN2 (TPCN2 Human)

Use

Tags

GCaMP6m

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-Tubulin-6

Plasmid

#56450

Purpose

Localization: Microtubules, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Tubulin (TUBA1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

Plasmid

#210523

Purpose

AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

GFP-KASH
U6-gRNA-directcapture

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-mito-Citron1

Plasmid

#134305

Purpose

mitochondria-targeted direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citron1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-flag-myr-Akt1-IRES-GFP

Plasmid

#65063

Purpose

mammalian retroviral expression plasmid

Depositor

Jean Zhao, Kira Gritsman

Article

Kharas et al Blood. 2010 Feb 18;115(7):1406-15. doi:

Insert

Akt1 (AKT1 Human)

Use

Tags

flag and myristoylation tag

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
piRFP670-N1

Plasmid

#45457

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

iRFP670

Use

Tags

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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R777-E209 Hs.RAC2

Plasmid

#70493

Purpose

Gateway ORF clone of human RAC2 [NM_002872.4] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RAC2 (RAC2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-TO-TEVP

Plasmid

#116062

Purpose

Expresses TEV protease

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

TEVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCT302

Plasmid

#41845

Depositor

Dane Wittrup

Article

Midelfort et al J Mol Biol. 2004 Oct 22;343(3):685-701.

Insert

4-4-20

Use

Tags

HA and c-myc

Expression

Yeast

Mutation

Promoter

Available since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

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TFORF2060

Plasmid

#141899

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGY53

Plasmid

#221709

Purpose

Empty Cas12a vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Article

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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