20,074 results

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pJL1-mRFP1

Plasmid

#102630

Purpose

In vitro expression of mRFP1 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mRFP1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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p5E-elavl3

Plasmid

#82400

Purpose

Gateway-compatible zebrafish elavl3 promoter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

elavl3 promoter

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAGT8163 (AATG_ttLbCas12a-i_TTCG)

Plasmid

#192453

Purpose

intronized ttLbCas12a for in planta expression

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)

Use

CRISPR and Synthetic Biology; Moclo compatible le…

Mutation

temperature-tolerant mutation (D156R)

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pQUAST-attB

Plasmid

#104880

Purpose

For insertion of 5xQUAS-geneX transgenes using PhiC31 integrase system

Depositor

Christopher Potter

Type

Empty backbone

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucSM EGFP

Plasmid

#90171

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q in FLuc

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-entry-puro

Plasmid

#85745

Purpose

Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pN1-GCaMP6m-XC

Plasmid

#111543

Purpose

Improved genetic encoded calcium sensor based on GCaMP6m. Also called GCaMP-X

Depositor

Xiaodong Liu

Article

Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

Insert

GCaMP6m-XC

Expression

Mammalian

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB405

Plasmid

#74799

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pKMyc

Plasmid

#19400

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Type

Empty backbone

Tags

Myc

Expression

Mammalian

Available Since

April 2, 2009

Availability

Academic Institutions and Nonprofits only

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pET His6 NusA TEV LIC cloning vector (1N)

Plasmid

#29657

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, NusA, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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mscv2.2-NLRC4

Plasmid

#60199

Purpose

Mouse NLRC4 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NLRC4

Depositor

Russell Vance

Article

Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

Insert

NLRC4 (Nlrc4 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1512

Plasmid

#62366

Purpose

N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pTRE2

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-tTA

Plasmid

#99119

Purpose

An AAV genome that expresses tTA from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

hSyn1

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pNLS-TetR-GFP

Plasmid

#103838

Purpose

Flourescent marker for tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

NLS-TetR (tetR )

Tags

EGFP

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-Cdc42

Plasmid

#12201

Depositor

Jonathan Chernoff

Article

Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

Insert

Cdc42 (CDC42 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pHCMV-VSVgSS-TM

Plasmid

#207320

Purpose

Allows cloning of protein coding sequences to be incorportated into envelope of virions

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP1

Plasmid

#22596

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP1 (USP1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-2NLS-Cas9-6XMS2

Plasmid

#166033

Purpose

Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

Depositor

Yujia Cai

Article

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Insert

SpCas9

Use

CRISPR and Lentiviral

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-ER-TurboID

Plasmid

#149415

Purpose

Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has Service

AAV8

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

TBG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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PB-TAC-ERP2

Plasmid

#80478

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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