We narrowed to 24,898 results for: CHI;

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pFETCh_ONECUT2

Plasmid

#135738

Purpose

Donor vector for 3' FLAG tag of human ONECUT2

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ONECUT2 Homology arms (ONECUT2 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode1

Plasmid

#83678

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode1

Use

Retroviral

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pPP378

Plasmid

#158797

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-1 (D371A)

Expression

Bacterial

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pQFT-lacZ

Plasmid

#208896

Purpose

pQFT with lacZ under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Insert

lacZ

Expression

Bacterial

Mutation

Contains two silent mutations and a missense muta…

Promoter

PQJ

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pFMD

Plasmid

#160921

Purpose

Formate dehydrogenase (FMD) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Formate dehydrogenase promoter

Use

Synthetic Biology

Mutation

BsaI and BbsI domesticated

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat18

Plasmid

#45146

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT18

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pBbE1k-2

Plasmid

#59945

Purpose

dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

Depositor

Taek Soon Lee

Article

Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

Inserts

dihydropterine reductase
pterin-4a-carbinolamine dehydratase

Promoter

pTrc

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR2-R2-PBR-L4

Plasmid

#141017

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR2-L3-PBL-R1

Plasmid

#141016

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTH849-gst-optec

Plasmid

#89590

Purpose

Plasmid for bacterial expression of His-tagged GST encoded by preferred codons

Depositor

Tobias von der Haar

Article

Jossé et al bioRxiv 200006

Insert

glutathione-S transferase codon-optimised for bacterial expression

Tags

8xhistidine

Expression

Bacterial

Promoter

T7

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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tdEos-Talin-18

Plasmid

#57672

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pDONR-P2rP4

Plasmid

#141015

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mYFP

Plasmid

#105144

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P81nmt1-mYFP

Tags

81nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDOC-K-glmS-lacIZ

Plasmid

#158061

Purpose

Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

Depositor

Mark Webber

Article

Holden et al Microbiology (Reading). 2020 Nov 23. doi

Insert

lacIZ operon

Use

Synthetic Biology

Expression

Bacterial

Promoter

lac

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8-cerulean3

Plasmid

#99358

Purpose

Bacterial expression of Cerulean3 fused to the 3' of cDNA of the fission yeast tropomyosin, cdc8.

Depositor

Dan Mulvihill

Article

Brooker et al FEBS Lett. 2016 Sep;590(18):3111-21. doi

Insert

cdc8 (cdc8 Fission Yeast)

Tags

Cerulean3 and Gly3-His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pATD

Plasmid

#141012

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

HSV-TK

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-zeoCV

Plasmid

#73890

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

zeoCV

Expression

Bacterial, Mammalian, and Y…

Promoter

CMV

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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BCJJ344

Plasmid

#117503

Purpose

UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtUBI10

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 4

Plasmid

#8474

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

391SQ392-PA (S391P, Q392A)

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pJK667

Plasmid

#71690

Purpose

Produces E. coli acetate kinase with an N-terminal His6 tag (H6AckA)

Depositor

T. J. Kappock

Article

Murphy et al Front Chem. 2016 May 23;4:23. doi: 10.33

Insert

acetate kinase (ackA Escherichia coli)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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