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pCG_SARS-CoV2 NSP1 C-V5-tag

Plasmid

#179972

Purpose

Mammalian expression vector for SARS-CoV-2 Nsp1, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-2 Nsp1 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT2 NCmut-msfGFP

Plasmid

#180320

Purpose

mammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT2 F20D, V27D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-BA.2-AVI

Plasmid

#184533

Purpose

Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-BA.2 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP Y58A in Artichoke

Plasmid

#169936

Purpose

Overexpression of BCL6-GFP Y58A mutant fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 Y58A (BCL6 Human)

Use

Lentiviral

Tags

GFP

Mutation

160R>C

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP R28A in Artichoke

Plasmid

#169933

Purpose

Overexpression of BCL6-GFP R28A mutant fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 R28A (BCL6 Human)

Use

Lentiviral

Tags

GFP

Mutation

160R>C

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

Plasmid

#64314

Purpose

chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

Depositor

Noriyuki Tsumaki

Article

Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

Inserts

COL11A2 promoter (COL11A2 Human)
COL11A2 enhancer (COL11A2 Human)

Use

Lentiviral

Tags

EGFP and IRES-PURO

Expression

Mammalian

Mutation

N/A

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-SK

Plasmid

#136577

Purpose

Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, Klf4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-T2A-Klf4 (Klf4, Sox2 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP1 C-V5-tag

Plasmid

#179961

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp1, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 Nsp1 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 NSP15 C-V5-tag

Plasmid

#179984

Purpose

Mammalian expression vector for RaTG13 Nsp15, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

RaTG13 Nsp15

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 M C-V5-tag

Plasmid

#179967

Purpose

Mammalian expression vector for SARS-CoV-1 M, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 M (M Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG_FBXL7 (R310H)

Plasmid

#171849

Purpose

pcDNA3-FLAG_FBXL7 (R310H)

Depositor

Michele Pagano

Article

Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

Insert

F-box and leucine rich repeat protein 7 (FBXL7 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Arginine 310 to Histidine

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-E484K-AVI

Plasmid

#176444

Purpose

Expresses SARS-CoV-2 RBD domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-E484K (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

E484K

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_9

Plasmid

#60258

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

NKX6-1 enhancer (NKX6-1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_25

Plasmid

#60305

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ROCK1 enhancer (ROCK1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERP2-KLF4

Plasmid

#192908

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of KLF4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

KLF4 (KLF4 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLE1 C-ISCmut

Plasmid

#160804

Purpose

Express C-terminal ISC mutant POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLE1 C-ISCmut (POLE Human)

Use

Retroviral

Tags

3xFLAG

Mutation

shPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

Plasmid

#173963

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif

Use

Luciferase

Mutation

1 mutated Coordinator motif

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pVHC

Plasmid

#112614

Purpose

promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Venus (H2BC3 Human, Aequorea victoria)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Promoterless vector, ready for promoter for mamma…

Tags

Venus

Expression

Mammalian

Promoter

No Promoter

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-SD1-L452R-T478K-AVI

Plasmid

#176456

Purpose

Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-SD1-L452R-T478K (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L452R, T478K

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-B.1.1.7-AVI

Plasmid

#176325

Purpose

Expresses SARS-CoV-2 S2P protein from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-B.1.1.7 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

69-70 del, 144del, N501Y, A570D, D614G, P681H, T7…

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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