We narrowed to 41,013 results for: Eras

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pCS2+-PIF6-EGFP

Plasmid

#154913

Purpose

1-100 amino acids of Arabidopsis PIF6 (Gene ID: 825382), tagged with EGFP fluorescent protein. Mammalian codon optimised.

Depositor

Clare Buckley, Jonathan Clarke

Article

Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

Insert

phytochrome interacting factor 3-like 2 (PIL2 Mustard Weed)

Use

Xenopus/avian/zebrafish

Tags

EGFP

Expression

Mammalian

Mutation

1-100 amino acids of Arabidopsis PIF6

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-EGFP-2A-Blast

Plasmid

#71215

Purpose

dCas9 fused to EGFP

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-EGFP

Use

Lentiviral

Tags

dSpCas9-EGFP

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

Plasmid

#197893

Purpose

Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

mCherry

Use

AAV

Mutation

N/A

Promoter

EF1a

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGV3341

Plasmid

#170279

Purpose

Expression of mutant HIV-2 RT p66 reverse transcriptase

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

HIV-2 RT p66 (mutant)

Expression

Bacterial

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Tet2 promoter F

Plasmid

#63879

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet2

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet2 Promoter fragment F

Use

Luciferase

Expression

Mammalian

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGV3340

Plasmid

#170278

Purpose

Expression of mutant HIV-2 RT p51 reverse transcriptase

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

HIV-2 RT p51 (mutant)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag GCN5 Y621A/P622A

Plasmid

#14425

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

GCN5 Y621A/P622A (KAT2A Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

Y621A, P622A. Catalytically inactive acetyltransf…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
ACE2(-202)-luc

Plasmid

#31112

Purpose

Luciferase reporter construct for human ACE2 promoter

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

ACE2 promoter (ACE2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -202 to +103

Available Since

Oct. 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-TRE-Gluc-RMA-IRES-EGFP

Plasmid

#189628

Purpose

Expresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Inserts

Gaussia luciferase fused to Fc
IRES-EGFP

Use

AAV and Luciferase

Tags

Gluc and IgG1 Fc

Expression

Mammalian

Promoter

IRES and TRE

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-ATRX ADD

Plasmid

#59698

Purpose

Contains histone modification interacting domain (ATRX ADD)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

ATRX ADD (ATRX Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP hBub1 K821R

Plasmid

#59813

Purpose

Allows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

Insert

Bub1 (BUB1 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

K821R

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-NL-GW

Plasmid

#113446

Purpose

NanoLuc Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

cmyc-NanoLuc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pF148 pSOD1G37RAcGFP1

Plasmid

#26409

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G37R

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-V5-Lem2

Plasmid

#120245

Purpose

Expresses V5-tagged Lem2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Lem2 (Lemd2 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GW-NL-myc

Plasmid

#113447

Purpose

NanoLuc Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

NanoLuc-cmyc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP hBub1 WT

Plasmid

#59812

Purpose

Allows the integration of GFP hBub1 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

Insert

Bub1 (BUB1 Human)

Tags

EmGFP

Expression

Mammalian

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRL-TK 3'FBXW7 UTR

Plasmid

#26649

Depositor

Alex Minella

Article

Xu et al J Biol Chem. 2010 Nov 5;285(45):34439-46

Insert

FBXW7 (FBXW7 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains only the 3'UTR of FBXW7, nucleotide…

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only
STK24_HUMAN_D0

Plasmid

#79742

Purpose

This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK24 (STK24 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
ePB-PURO-FLAG-METTL3

Plasmid

#160252

Purpose

For stable integration of FLAG-METTL3 in human cells with trasposase

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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1037 pGL3 FasL promoter

Plasmid

#9028

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

Fas ligand promoter (FASLG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only