We narrowed to 16,582 results for: EMB

1. pSBinit

   Plasmid

   #110100

   Purpose

   E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTag

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Type

   Empty backbone

   Use

   Tags

   myc, His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-FLAG-Bbs4

   Plasmid

   #128868

   Purpose

   For mammalian expression of mouse Bbs4

   Depositor

   Aimin Liu

   Article

   Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

   Insert

   Bbs4 (Bbs4 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5HT6-YFP-PIPK(D253A)

   Plasmid

   #96811

   Purpose

   kinase-dead control for 5HT6-YFP-PIPK

   Depositor

   Takanari Inoue

   Article

   Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

   Insert

   5HT6-YFP-PIPK(D253A)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   D253A inactivates kinase activity of PIPK

   Promoter

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. iRFP-Sec61beta

   Plasmid

   #108125

   Purpose

   ER marker

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   iRFP:SEC61B (SEC61B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEGFP-NbALFA

   Plasmid

   #159986

   Purpose

   For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   mEGFP-NbALFA
   

   Use

   Tags

   8histidine-HRV3C protease cleavage site

   Expression

   Bacterial

   Mutation

   3GS linker between mEGFP and NbALFA

   Promoter

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-GalT

   Plasmid

   #11929

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Cole et al Science. 1996 Aug 9. 273(5276):797-801.

   Insert

   GalT (GALT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 1-60 of the human galactosyltransfera…

   Promoter

   Available since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. AA041

   Plasmid

   #215963

   Purpose

   Fragmid fragment: (Pol 2 promoter) human core (short) EF1a promoter

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   EFS_v1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AA294

   Plasmid

   #215968

   Purpose

   Fragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoter

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U1a_v1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA332

   Plasmid

   #216053

   Purpose

   Fragmid fragment: (C' terminus) 3x NLS sequences

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   SV40NLS_v1.10; SV40NLS_v1.11; SV40NLS_v1.12
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBITE-EGFRvIII-F269-OKT3

    Plasmid

    #190681

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-c-Myc-T58A

    Plasmid

    #13372

    Purpose

    Retroviral expression of mouse c-Myc T58A mutant

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    myelocytomatosis oncogene (T58A mutant) (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Tyrosine 58 to Alanine

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-CD63-(TEV)-tTA

    Plasmid

    #162597

    Purpose

    human CD63-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD63 (CD63 Human)
    

    Use

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFX-mCherry-KRasCT

    Plasmid

    #175835

    Purpose

    Express mCherry with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with plasma membrane-targeting sequence
    

    Use

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMVTNT-T7-KPNA2

    Plasmid

    #26678

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 . 11():63.

    Insert

    Karyopherin Alpha 2 (KPNA2 Human)
    

    Use

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMVTNT-T7-KPNA1

    Plasmid

    #26677

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 . 11():63.

    Insert

    Karyopherin Alpha 1 (KPNA1 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    KPNA1 sequence contains a reported SNP at S73N

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. UAS:mCherry

    Plasmid

    #170994

    Purpose

    UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    UAS:mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synthetic 14x UAS:Gal4

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac(His10-Lckg2a)

    Plasmid

    #177891

    Purpose

    Baculo expression of His10 tag fused to human LckG2A

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Lck (LCK Human)
    

    Use

    Tags

    10xHis-TEV

    Expression

    Insect

    Mutation

    G2A

    Promoter

    Polyhedrin

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCh-Sec61 beta

    Plasmid

    #49155

    Purpose

    expression of Sec61 beta fused to mCherry, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    mCherry*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-p97

    Plasmid

    #85670

    Purpose

    Expression of p97 with N-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    p97 (Vcp Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMVHA E2F1

    Plasmid

    #24225

    Purpose

    Mammalian expression of human E2F1 with HA tag

    Depositor

    Kristian Helin

    Article

    Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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