We narrowed to 65,914 results for: des.1
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Plasmid#208045PurposeEnables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycinDepositorAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only
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QUIN
Plasmid#184267PurposeA binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.DepositorInsertCPSMV RNA1 cDNA with 4 introns
ExpressionPlantMutationS1098P, K1651RPromoterCaMV 35S with duplicated enhancerAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Zscan4 promoter
Plasmid#69249PurposeLuciferase reporter for Dux4 activation of Zscan4 transcriptionDepositorInsertZscan4 (ZSCAN4 Human)
UseLuciferaseMutationThe two single nucleotide mismatches found betwee…PromoterZscan4Available SinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBR-Cas9ΔyqhD-HA500
Plasmid#220369PurposeA single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440DepositorInsertsSpCas9
sgRNA for yqhD in P. putida KT2440
UseCRISPR; Broad host rangeExpressionBacterialAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-3-SARS-CoV-2-3CL
Plasmid#168457PurposeBacterial codon optimized expression vector for SARS-CoV-2 3CL protease.DepositorAvailable SinceMarch 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-TRE-tdTomato-WPRE
Plasmid#104112PurposeAAV-mediated expression of tdTomato under the TRE promoter.DepositorInserttdTomato
UseAAVPromoterTREAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
FZ-NbPDS
Plasmid#184268PurposepAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.DepositorInsertCPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
ExpressionPlantMutationThe protease processing site between MP and L-CP …PromoterCaMV 35S with duplicated enhancerAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFD116
Plasmid#124769PurposepFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
dcas9
PpflB sgRNA BsaI
UseCRISPRAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Plasmid#62373PurposeExpression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cellsDepositorInsert(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Tags(-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…ExpressionBacterialMutationY67S amino acid substitution in (-30)GFP renders …PromoterT7Available SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP618
Plasmid#169235PurposeSpear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adaptersDepositorInsertmU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-PdCO-mScarlet-WPRE
Plasmid#198510PurposeExpresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor.DepositorHas ServiceAAV1 and AAV5InsertPdCO
UseAAVTagsRho1D4 and mScarletExpressionMammalianPromoterhSyn1Available SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-JEDI-2P-Kv-WPRE
Plasmid#179463PurposeSoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSynDepositorHas ServiceAAV9InsertJEDI-2P-Kv
UseAAVExpressionMammalianPromoterhSynAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-EYFP
Plasmid#137162PurposeIntersectional viral expression of EYFP in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationn/aPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s
Plasmid#105715PurposeIn the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.DepositorHas ServiceAAV5Insertsynaptophysin-GCaMP6s
UseAAV and Cre/LoxExpressionMammalianPromoterEf1a, Human elongation factor-1 alphaAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PKAKTRClover
Plasmid#59146PurposeORF encoding PKA KTR mClover flanked by gateway sequencesDepositorInsertPKA Kinase Translocation Reporter (PRKACA Human, Mouse)
Available SinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRDA_336
Plasmid#179095PurposeBE Cas expressionDepositorInsertBE3.9 (Cas9-NG)
UseCRISPR and LentiviralAvailable SinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_sfGFP1-10
Plasmid#153515PurposeE.coli expression plasmid for the production of GFP1-10DepositorInsertsfGFP1-10
ExpressionBacterialAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTet
Plasmid#124663PurposeExpresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coilDepositorInsertSpyCatcher002-oTet
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH-PABE-7-esgRNA
Plasmid#115620PurposeTargeted A to G in riceDepositorInsertwtTadA-TadA7.10-nCas9-3*NLS
UseCRISPRExpressionPlantAvailable SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only